Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coliderived recombinant human bone morphogenetic protein 2 in rat calvarial defects

Jin Woo Kim, Kyung Hee Choi, Jeong Ho Yun, Ui Won Jung, Chang Sung Kim, Seong Ho Choi, Kyoo Sung Cho

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35 Citations (Scopus)

Abstract

The objective of this study was to evaluate bone formation in rat calvarial defects after surgical implantation of block or particulated biphasic calcium phosphate (BCP) lyophilized with Escherichia coliderived recombinant human bone morphogenetic protein 2 (ErhBMP-2). Critical-size calvarial osteotomy defects were created in 5 groups of Sprague-Dawley rats. Each group received one of the following: 1) sham surgery control; 2) biphasic calcium phosphate particles (CPP); 3) biphasic calcium phosphate block (CPB); 4) ErhBMP-2coated CPP; or 5) ErhBMP-2coated CPB. ErhBMP was coated on BCP by a stepwise lyophilizing protocol. The new bone formation was significantly greater in ErhBMP-2treated groups compared with the untreated group. In particular, the ErhBMP-2/CPB group showed stability of augmented areas during the period of healing, due to relevant space-providing capacity. Thus, it can be concluded that CPP and CPB lyophilized with ErhBMP-2 enhance the formation of new bone, and CPB appears to be a suitable carrier for ErhBMP-2 in which a 3-dimensional structural integrity is an important consideration factor.

Original languageEnglish
Pages (from-to)298-306
Number of pages9
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume112
Issue number3
DOIs
Publication statusPublished - 2011 Sep 1

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Escherichia
Osteogenesis
Osteotomy
Sprague Dawley Rats
recombinant human bone morphogenetic protein-2
hydroxyapatite-beta tricalcium phosphate
calcium phosphate

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

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title = "Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coliderived recombinant human bone morphogenetic protein 2 in rat calvarial defects",
abstract = "The objective of this study was to evaluate bone formation in rat calvarial defects after surgical implantation of block or particulated biphasic calcium phosphate (BCP) lyophilized with Escherichia coliderived recombinant human bone morphogenetic protein 2 (ErhBMP-2). Critical-size calvarial osteotomy defects were created in 5 groups of Sprague-Dawley rats. Each group received one of the following: 1) sham surgery control; 2) biphasic calcium phosphate particles (CPP); 3) biphasic calcium phosphate block (CPB); 4) ErhBMP-2coated CPP; or 5) ErhBMP-2coated CPB. ErhBMP was coated on BCP by a stepwise lyophilizing protocol. The new bone formation was significantly greater in ErhBMP-2treated groups compared with the untreated group. In particular, the ErhBMP-2/CPB group showed stability of augmented areas during the period of healing, due to relevant space-providing capacity. Thus, it can be concluded that CPP and CPB lyophilized with ErhBMP-2 enhance the formation of new bone, and CPB appears to be a suitable carrier for ErhBMP-2 in which a 3-dimensional structural integrity is an important consideration factor.",
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AU - Jung, Ui Won

AU - Kim, Chang Sung

AU - Choi, Seong Ho

AU - Cho, Kyoo Sung

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