Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects

Jee Hee Jung, Jeong Ho Yun, Yoo Jung Um, Ui-Won Jung, ChangSung Kim, Seongho Choi, Kyoo Sung Cho

Research output: Contribution to journalArticle

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Abstract

Objectives: The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model. Study design: Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals. Results: On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio (P < .05). Conclusion: ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2.

Original languageEnglish
Pages (from-to)298-305
Number of pages8
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume111
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

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Osteogenesis
Collagen
Escherichia coli
Bone and Bones
Bone Regeneration
Sprague Dawley Rats
Observation

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

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title = "Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects",
abstract = "Objectives: The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model. Study design: Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals. Results: On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio (P < .05). Conclusion: ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2.",
author = "Jung, {Jee Hee} and Yun, {Jeong Ho} and Um, {Yoo Jung} and Ui-Won Jung and ChangSung Kim and Seongho Choi and Cho, {Kyoo Sung}",
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Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects. / Jung, Jee Hee; Yun, Jeong Ho; Um, Yoo Jung; Jung, Ui-Won; Kim, ChangSung; Choi, Seongho; Cho, Kyoo Sung.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, Vol. 111, No. 3, 01.03.2011, p. 298-305.

Research output: Contribution to journalArticle

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AU - Jung, Jee Hee

AU - Yun, Jeong Ho

AU - Um, Yoo Jung

AU - Jung, Ui-Won

AU - Kim, ChangSung

AU - Choi, Seongho

AU - Cho, Kyoo Sung

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AB - Objectives: The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model. Study design: Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals. Results: On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio (P < .05). Conclusion: ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2.

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