Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy

Hyongbum Kim, Jong Seon Park, Jin Choi Yong, Mee Ohk Kim, Hoon Huh Yang, Sung Whan Kim, Woong Han Ji, Ji Yoon Lee, Sinae Kim, Mackenzie A. Houge, Masaaki Ii, Youngsup Yoon

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Bone marrow-derived mononuclear cells (BMNCs) have been shown to effectively treat ischemic cardiovascular diseases. Because diabetic neuropathy (DN) is causally associated with impaired angiogenesis and deficiency of angiogenic and neurotrophic factors in the nerves, we investigated whether DN can be ameliorated by local injection of BMNCs. Severe peripheral neuropathy, characterized by a significant decrease in the motor and sensory nerve conduction velocities (NCVs), developed 12 weeks after the induction of diabetes with streptozotocin in rats. The injection of BMNCs restored motor and sensory NCVs to normal levels and significantly improved vascular density and blood flow in diabetic nerves over 4 weeks. Fluorescent microscopic observation revealed that DiI-labeled BMNCs preferentially engrafted in sciatic nerves. Whole-mount fluorescent imaging and confocal microscopic evaluation demonstrated that many of the BMNCs localized following the course of the vasa nervorum in close proximity to blood vessels without incorporation into vasa nervorum as endothelial cells at a detectable level. Real-time reverse transcription- polymerase chain reaction analysis showed that the levels of angiogenic and neurotrophic factors were significantly increased in the nerves by BMNC injection. Local transplantation of BMNCs improved experimental DN by augmenting angiogenesis and increasing angiogenic and neurotrophic factors in peripheral nerves. These findings suggest that BMNC transplantation may represent a novel therapeutic option for treating DN.

Original languageEnglish
Pages (from-to)1686-1696
Number of pages11
JournalStem Cells
Volume27
Issue number7
DOIs
Publication statusPublished - 2009 Jul 1

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Tropism
Diabetic Neuropathies
Bone Marrow Cells
Bone Marrow
Angiogenesis Inducing Agents
Vasa Nervorum
Nerve Growth Factors
Neural Conduction
Injections
Blood Vessels
Experimental Diabetes Mellitus
Cell Transplantation
Peripheral Nervous System Diseases
Sciatic Nerve
Bone Marrow Transplantation
Peripheral Nerves
Reverse Transcription
Cardiovascular Diseases
Endothelial Cells
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Kim, H., Park, J. S., Yong, J. C., Kim, M. O., Yang, H. H., Kim, S. W., ... Yoon, Y. (2009). Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy. Stem Cells, 27(7), 1686-1696. https://doi.org/10.1002/stem.87
Kim, Hyongbum ; Park, Jong Seon ; Yong, Jin Choi ; Kim, Mee Ohk ; Yang, Hoon Huh ; Kim, Sung Whan ; Ji, Woong Han ; Lee, Ji Yoon ; Kim, Sinae ; Houge, Mackenzie A. ; Ii, Masaaki ; Yoon, Youngsup. / Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy. In: Stem Cells. 2009 ; Vol. 27, No. 7. pp. 1686-1696.
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Kim, H, Park, JS, Yong, JC, Kim, MO, Yang, HH, Kim, SW, Ji, WH, Lee, JY, Kim, S, Houge, MA, Ii, M & Yoon, Y 2009, 'Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy', Stem Cells, vol. 27, no. 7, pp. 1686-1696. https://doi.org/10.1002/stem.87

Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy. / Kim, Hyongbum; Park, Jong Seon; Yong, Jin Choi; Kim, Mee Ohk; Yang, Hoon Huh; Kim, Sung Whan; Ji, Woong Han; Lee, Ji Yoon; Kim, Sinae; Houge, Mackenzie A.; Ii, Masaaki; Yoon, Youngsup.

In: Stem Cells, Vol. 27, No. 7, 01.07.2009, p. 1686-1696.

Research output: Contribution to journalArticle

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Kim H, Park JS, Yong JC, Kim MO, Yang HH, Kim SW et al. Bone marrow mononuclear cells have neurovascular tropism and improve diabetic neuropathy. Stem Cells. 2009 Jul 1;27(7):1686-1696. https://doi.org/10.1002/stem.87