Bone marrow mononuclear stem cells transplanted in rat enfarct myocardium improved the electrical conduction without evidence of proarrhythmic effects

Boyoung Joung, Il Kwon Kim, Moon Hyoung Lee, Kyung Jong Yoo, Sung Soon Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: The arrhythmogenic effect of stem cells transplantation (SCT) in an infarct myocardium is still unknown. We investigated arrhythmogenicity of SCT in rat cryo-infarct model. Materials and Methods: In rat cryo-infarct model, bone marrow mononuclear stem cells (MNSC, 1 × 107 cells) were transplanted into the infarct border zone (BZ) of the LV epicardium. We compared the optical mapping and inducibility of ventricular tachycardia/ fibrillation (VT/VF) among normal (n = 5), cryo-infarct (n = 6), and SCT rats (n = 6). Results: The VT/VF inducibility was higher in the cryo-infarct (47.2%, p = 0.001) and SCT groups (34.6%, p = 0.01) than in the normal group (12.8%). The induced VT/VF episodes persisted for more than 2 minutes in 4.3%, 26.4% and 17.3% in the normal, cryo-infarct and SCT group, respectively. In the SCT group, the action potential duration at 70% was shorter at the SCT site than the BZ during SR (75.2 ± 8.1 vs. 145.6 ± 4.4 ms, p = 0.001) and VT (78.2 ± 13.0 vs. 125.7 ± 21.0 ms, p = 0.001). Conduction block was observed at the SCT site and BZ during VT. However, no reentry or ectopic foci were observed around the SCT sites. Conclusion: The electrical conduction was improved by SCT without evidence of augmentation of arrhythmia in the rat cryo-infarct model.

Original languageEnglish
Pages (from-to)754-764
Number of pages11
JournalYonsei medical journal
Volume48
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Stem Cell Transplantation
Myocardium
Stem Cells
Bone Marrow
Pericardium
Ventricular Fibrillation
Ventricular Tachycardia
Action Potentials
Cardiac Arrhythmias

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{0280bee980654f41b99f5dcd5d5c28ad,
title = "Bone marrow mononuclear stem cells transplanted in rat enfarct myocardium improved the electrical conduction without evidence of proarrhythmic effects",
abstract = "Purpose: The arrhythmogenic effect of stem cells transplantation (SCT) in an infarct myocardium is still unknown. We investigated arrhythmogenicity of SCT in rat cryo-infarct model. Materials and Methods: In rat cryo-infarct model, bone marrow mononuclear stem cells (MNSC, 1 × 107 cells) were transplanted into the infarct border zone (BZ) of the LV epicardium. We compared the optical mapping and inducibility of ventricular tachycardia/ fibrillation (VT/VF) among normal (n = 5), cryo-infarct (n = 6), and SCT rats (n = 6). Results: The VT/VF inducibility was higher in the cryo-infarct (47.2{\%}, p = 0.001) and SCT groups (34.6{\%}, p = 0.01) than in the normal group (12.8{\%}). The induced VT/VF episodes persisted for more than 2 minutes in 4.3{\%}, 26.4{\%} and 17.3{\%} in the normal, cryo-infarct and SCT group, respectively. In the SCT group, the action potential duration at 70{\%} was shorter at the SCT site than the BZ during SR (75.2 ± 8.1 vs. 145.6 ± 4.4 ms, p = 0.001) and VT (78.2 ± 13.0 vs. 125.7 ± 21.0 ms, p = 0.001). Conduction block was observed at the SCT site and BZ during VT. However, no reentry or ectopic foci were observed around the SCT sites. Conclusion: The electrical conduction was improved by SCT without evidence of augmentation of arrhythmia in the rat cryo-infarct model.",
author = "Boyoung Joung and Kim, {Il Kwon} and Lee, {Moon Hyoung} and Yoo, {Kyung Jong} and Kim, {Sung Soon}",
year = "2007",
month = "10",
day = "1",
doi = "10.3349/ymj.2007.48.5.754",
language = "English",
volume = "48",
pages = "754--764",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "5",

}

Bone marrow mononuclear stem cells transplanted in rat enfarct myocardium improved the electrical conduction without evidence of proarrhythmic effects. / Joung, Boyoung; Kim, Il Kwon; Lee, Moon Hyoung; Yoo, Kyung Jong; Kim, Sung Soon.

In: Yonsei medical journal, Vol. 48, No. 5, 01.10.2007, p. 754-764.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bone marrow mononuclear stem cells transplanted in rat enfarct myocardium improved the electrical conduction without evidence of proarrhythmic effects

AU - Joung, Boyoung

AU - Kim, Il Kwon

AU - Lee, Moon Hyoung

AU - Yoo, Kyung Jong

AU - Kim, Sung Soon

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Purpose: The arrhythmogenic effect of stem cells transplantation (SCT) in an infarct myocardium is still unknown. We investigated arrhythmogenicity of SCT in rat cryo-infarct model. Materials and Methods: In rat cryo-infarct model, bone marrow mononuclear stem cells (MNSC, 1 × 107 cells) were transplanted into the infarct border zone (BZ) of the LV epicardium. We compared the optical mapping and inducibility of ventricular tachycardia/ fibrillation (VT/VF) among normal (n = 5), cryo-infarct (n = 6), and SCT rats (n = 6). Results: The VT/VF inducibility was higher in the cryo-infarct (47.2%, p = 0.001) and SCT groups (34.6%, p = 0.01) than in the normal group (12.8%). The induced VT/VF episodes persisted for more than 2 minutes in 4.3%, 26.4% and 17.3% in the normal, cryo-infarct and SCT group, respectively. In the SCT group, the action potential duration at 70% was shorter at the SCT site than the BZ during SR (75.2 ± 8.1 vs. 145.6 ± 4.4 ms, p = 0.001) and VT (78.2 ± 13.0 vs. 125.7 ± 21.0 ms, p = 0.001). Conduction block was observed at the SCT site and BZ during VT. However, no reentry or ectopic foci were observed around the SCT sites. Conclusion: The electrical conduction was improved by SCT without evidence of augmentation of arrhythmia in the rat cryo-infarct model.

AB - Purpose: The arrhythmogenic effect of stem cells transplantation (SCT) in an infarct myocardium is still unknown. We investigated arrhythmogenicity of SCT in rat cryo-infarct model. Materials and Methods: In rat cryo-infarct model, bone marrow mononuclear stem cells (MNSC, 1 × 107 cells) were transplanted into the infarct border zone (BZ) of the LV epicardium. We compared the optical mapping and inducibility of ventricular tachycardia/ fibrillation (VT/VF) among normal (n = 5), cryo-infarct (n = 6), and SCT rats (n = 6). Results: The VT/VF inducibility was higher in the cryo-infarct (47.2%, p = 0.001) and SCT groups (34.6%, p = 0.01) than in the normal group (12.8%). The induced VT/VF episodes persisted for more than 2 minutes in 4.3%, 26.4% and 17.3% in the normal, cryo-infarct and SCT group, respectively. In the SCT group, the action potential duration at 70% was shorter at the SCT site than the BZ during SR (75.2 ± 8.1 vs. 145.6 ± 4.4 ms, p = 0.001) and VT (78.2 ± 13.0 vs. 125.7 ± 21.0 ms, p = 0.001). Conduction block was observed at the SCT site and BZ during VT. However, no reentry or ectopic foci were observed around the SCT sites. Conclusion: The electrical conduction was improved by SCT without evidence of augmentation of arrhythmia in the rat cryo-infarct model.

UR - http://www.scopus.com/inward/record.url?scp=36249019756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36249019756&partnerID=8YFLogxK

U2 - 10.3349/ymj.2007.48.5.754

DO - 10.3349/ymj.2007.48.5.754

M3 - Article

C2 - 17963331

AN - SCOPUS:36249019756

VL - 48

SP - 754

EP - 764

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 5

ER -