Bone regenerative efficacy of limited-dose Escherichia coli-derived rhBMP-2 with biphasic calcium phosphate carrier in rabbit calvarial defect model

Hoon You, So Ra Yoon, Hyun Chang Lim, Jung Seok Lee, Ui Won Jung, Seong Ho Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 g) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier. Materials and Methods: Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses. Results: Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing. Conclusion: ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 g). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalImplant Dentistry
Volume25
Issue number1
DOIs
Publication statusPublished - 2016 Feb 1

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Phosphate Transport Proteins
Porifera
Escherichia coli
Rabbits
Bone and Bones
Collagen
Osteogenesis
X-Ray Microtomography
Bone Regeneration
Carrier Proteins
hydroxyapatite-beta tricalcium phosphate

All Science Journal Classification (ASJC) codes

  • Oral Surgery

Cite this

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title = "Bone regenerative efficacy of limited-dose Escherichia coli-derived rhBMP-2 with biphasic calcium phosphate carrier in rabbit calvarial defect model",
abstract = "Purpose: The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 g) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier. Materials and Methods: Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses. Results: Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing. Conclusion: ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 g). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.",
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Bone regenerative efficacy of limited-dose Escherichia coli-derived rhBMP-2 with biphasic calcium phosphate carrier in rabbit calvarial defect model. / You, Hoon; Yoon, So Ra; Lim, Hyun Chang; Lee, Jung Seok; Jung, Ui Won; Choi, Seong Ho.

In: Implant Dentistry, Vol. 25, No. 1, 01.02.2016, p. 16-23.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bone regenerative efficacy of limited-dose Escherichia coli-derived rhBMP-2 with biphasic calcium phosphate carrier in rabbit calvarial defect model

AU - You, Hoon

AU - Yoon, So Ra

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AU - Lee, Jung Seok

AU - Jung, Ui Won

AU - Choi, Seong Ho

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N2 - Purpose: The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 g) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier. Materials and Methods: Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses. Results: Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing. Conclusion: ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 g). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.

AB - Purpose: The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 g) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier. Materials and Methods: Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses. Results: Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing. Conclusion: ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 g). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.

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