Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin–Biotin Binding System

Min Chul Kim, Min Ho Hong, Byung Hyun Lee, Heon-Jin Choi, Yeong Mu Ko, Yong Keun Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Highly porous and interconnected scaffolds were fabricated using calcium phosphate glass (CPG) for bone tissue engineering. An avidin–biotin binding system was used to improve osteoblast-like cell adhesion to the scaffold. The scaffolds had open macro- and micro-scale pores, and continuous struts without cracks or defects. Scaffolds prepared using a mixture (amorphous and crystalline CPG) were stronger than amorphous group and crystalline group. Cell adhesion assays showed that more cells adhered, with increasing cell seeding efficiency to the avidin-adsorbed scaffolds, and that cell attachment to the highly porous scaffolds significantly differed between avidin-adsorbed scaffolds and other scaffolds. Proliferation was also significantly higher for avidin-adsorbed scaffolds. Osteoblastic differentiation of MG-63 cells was observed at 3 days, and MG-63 cells in direct contact with avidin-adsorbed scaffolds were positive for type I collagen, osteopontin, and alkaline phosphatase gene expression. Osteocalcin expression was observed in the avidin-adsorbed scaffolds at 7 days, indicating that cell differentiation in avidin-adsorbed scaffolds occurred faster than the other scaffolds. Thus, these CPG scaffolds have excellent biological properties suitable for use in bone tissue engineering.

Original languageEnglish
Pages (from-to)3004-3014
Number of pages11
JournalAnnals of Biomedical Engineering
Volume43
Issue number12
DOIs
Publication statusPublished - 2015 Jun 4

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Calcium phosphate
Scaffolds (biology)
Tissue engineering
Scaffolds
Bone
Glass
Cell adhesion
Crystalline materials
Struts
Osteoblasts
Phosphatases
Collagen
Gene expression
Macros
Assays
Cracks
Defects

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

Kim, Min Chul ; Hong, Min Ho ; Lee, Byung Hyun ; Choi, Heon-Jin ; Ko, Yeong Mu ; Lee, Yong Keun. / Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin–Biotin Binding System. In: Annals of Biomedical Engineering. 2015 ; Vol. 43, No. 12. pp. 3004-3014.
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Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin–Biotin Binding System. / Kim, Min Chul; Hong, Min Ho; Lee, Byung Hyun; Choi, Heon-Jin; Ko, Yeong Mu; Lee, Yong Keun.

In: Annals of Biomedical Engineering, Vol. 43, No. 12, 04.06.2015, p. 3004-3014.

Research output: Contribution to journalArticle

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