Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland

Identification of a bacterial strain, designated CJ71^T, was carried out using a polyphasic taxonomic approach. Strain CJ71^T was isolated from sediment from the estuarine wetland of the Han River, South Korea, by enrichment culture using pyrene as the sole carbon and energy source. The isolate was white-pigmented, rod-shaped, Gram-positive, strictly aerobic and motile. 16S rRNA gene sequence analysis revealed that strain CJ71^T had the highest sequence similarity (96.9%) to Brevibacillus formosus DSM 9885^T. The predominant cellular fatty acids in strain CJ71 ^T were anteiso-C_{15 : 0} (49.5%), iso-C_{15 : 0} (16.9%), iso-C_{14 : 0} (16.9%) and iso-C_{16 : 0} (4.9 %). The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 52.4 mol%. Results from the polyphasic taxonomic study suggest that strain CJ71 ^T represents a novel species of the genus Brevibacillus for which the name Brevibacillus fluminis sp. nov. is proposed; the type strain is CJ71 ^T (=KACC 13381^T=JCM 15716^T)