Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture

Yingshun Cui, Myung Suk Kang, Sung Geun Woo, Long Jin, Kwang Kyu Kim, Joonhong Park, Myungjin Lee, Sung Taik Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2%), Brevibacterium salitolerans KCTC 19616T (96.7%), Brevibacterium album KCTC 19173T (96.2%) and Brevibacterium samyangense KCCM 42316T (96.2%). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15: 0, anteiso-C17: 0, C16: 0 and iso-C15: 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T (5KCTC 19800T5JCM 17458T).

Original languageEnglish
Pages (from-to)152-157
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue number1
DOIs
Publication statusPublished - 2013 Jan 23

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Brevibacterium
chlorophenols
enrichment culture
chlorophenol
Nitrates
nitrates
nitrate
Bacteria
DNA
bacterium
new species
bacteria
phylogenetics
acid
relatedness
cleavage
activated sludge
catechol
sugar
glucose

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Cui, Yingshun ; Kang, Myung Suk ; Woo, Sung Geun ; Jin, Long ; Kim, Kwang Kyu ; Park, Joonhong ; Lee, Myungjin ; Lee, Sung Taik. / Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. 1. pp. 152-157.
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abstract = "A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2{\%}), Brevibacterium salitolerans KCTC 19616T (96.7{\%}), Brevibacterium album KCTC 19173T (96.2{\%}) and Brevibacterium samyangense KCCM 42316T (96.2{\%}). The DNA G+C content of strain 2C6-41T was 66.4 mol{\%}. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15: 0, anteiso-C17: 0, C16: 0 and iso-C15: 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T (5KCTC 19800T5JCM 17458T).",
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Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. / Cui, Yingshun; Kang, Myung Suk; Woo, Sung Geun; Jin, Long; Kim, Kwang Kyu; Park, Joonhong; Lee, Myungjin; Lee, Sung Taik.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. 1, 23.01.2013, p. 152-157.

Research output: Contribution to journalArticle

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T1 - Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture

AU - Cui, Yingshun

AU - Kang, Myung Suk

AU - Woo, Sung Geun

AU - Jin, Long

AU - Kim, Kwang Kyu

AU - Park, Joonhong

AU - Lee, Myungjin

AU - Lee, Sung Taik

PY - 2013/1/23

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N2 - A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2%), Brevibacterium salitolerans KCTC 19616T (96.7%), Brevibacterium album KCTC 19173T (96.2%) and Brevibacterium samyangense KCCM 42316T (96.2%). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15: 0, anteiso-C17: 0, C16: 0 and iso-C15: 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T (5KCTC 19800T5JCM 17458T).

AB - A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2%), Brevibacterium salitolerans KCTC 19616T (96.7%), Brevibacterium album KCTC 19173T (96.2%) and Brevibacterium samyangense KCCM 42316T (96.2%). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15: 0, anteiso-C17: 0, C16: 0 and iso-C15: 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T (5KCTC 19800T5JCM 17458T).

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