BRI1-Associated Receptor Kinase 1 Regulates Guard Cell ABA Signaling Mediated by Open Stomata 1 in Arabidopsis

Yun Shang; Changbo Dai; Myeong Min Lee; June M. Kwak; Kyoung Hee Nam

doi:10.1016/j.molp.2015.12.014

BRI1-Associated Receptor Kinase 1 Regulates Guard Cell ABA Signaling Mediated by Open Stomata 1 in Arabidopsis

Yun Shang, Changbo Dai, Myeong Min Lee, June M. Kwak, Kyoung Hee Nam

Research output: Contribution to journal › Article › peer-review

112 Citations (Scopus)

Abstract

Stomatal movements are critical in regulating gas exchange for photosynthesis and water balance between plant tissues and the atmosphere. The plant hormone abscisic acid (ABA) plays key roles in regulating stomatal closure under various abiotic stresses. In this study, we revealed a novel role of BAK1 in guard cell ABA signaling. We found that the brassinosteroid (BR) signaling mutant bak1 lost more water than wild-type plants and showed ABA insensitivity in stomatal closure. ABA-induced OST1 expression and reactive oxygen species (ROS) production were also impaired in bak1. Unlike direct treatment with H₂O₂, overexpression of OST1 did not completely rescue the insensitivity of bak1 to ABA. We demonstrated that BAK1 forms a complex with OST1 near the plasma membrane and that the BAK1/OST1 complex is increased in response to ABA in planta. Brassinolide, the most active BR, exerted a negative effect on ABA-induced formation of the BAK1/OST1 complex and OST1 expression. Moreover, we found that BAK1 and ABI1 oppositely regulate OST1 phosphorylation in vitro, and that ABI1 interacts with BAK1 and inhibits the interaction of BAK1 and OST1. Taken together, our results suggest that BAK1 regulates ABA-induced stomatal closure in guard cells.

Original language	English
Pages (from-to)	447-460
Number of pages	14
Journal	Molecular Plant
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/j.molp.2015.12.014
Publication status	Published - 2016 Mar 7

Bibliographical note

Funding Information:
This work was supported by grants from the Next-Generation BioGreen 21 Program ( SSAC , PJ011059 to K.H.N.), Rural Development Administration , Republic of Korea, and the SRC Research Center for Women's Diseases of Sookmyung Women's University (2011) (grant # 3-1103-0020 to K.H.N.), and by the National Research Foundation ( NRF-2012R1A2A2A01009953 to M.M.L.), and in part by project IBS-R013-G2-2014-a00 to J.M.K.

Publisher Copyright:
© 2016 The Author.

All Science Journal Classification (ASJC) codes

Molecular Biology
Plant Science

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10.1016/j.molp.2015.12.014

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title = "BRI1-Associated Receptor Kinase 1 Regulates Guard Cell ABA Signaling Mediated by Open Stomata 1 in Arabidopsis",

abstract = "Stomatal movements are critical in regulating gas exchange for photosynthesis and water balance between plant tissues and the atmosphere. The plant hormone abscisic acid (ABA) plays key roles in regulating stomatal closure under various abiotic stresses. In this study, we revealed a novel role of BAK1 in guard cell ABA signaling. We found that the brassinosteroid (BR) signaling mutant bak1 lost more water than wild-type plants and showed ABA insensitivity in stomatal closure. ABA-induced OST1 expression and reactive oxygen species (ROS) production were also impaired in bak1. Unlike direct treatment with H2O2, overexpression of OST1 did not completely rescue the insensitivity of bak1 to ABA. We demonstrated that BAK1 forms a complex with OST1 near the plasma membrane and that the BAK1/OST1 complex is increased in response to ABA in planta. Brassinolide, the most active BR, exerted a negative effect on ABA-induced formation of the BAK1/OST1 complex and OST1 expression. Moreover, we found that BAK1 and ABI1 oppositely regulate OST1 phosphorylation in vitro, and that ABI1 interacts with BAK1 and inhibits the interaction of BAK1 and OST1. Taken together, our results suggest that BAK1 regulates ABA-induced stomatal closure in guard cells.",

author = "Yun Shang and Changbo Dai and Lee, {Myeong Min} and Kwak, {June M.} and Nam, {Kyoung Hee}",

note = "Funding Information: This work was supported by grants from the Next-Generation BioGreen 21 Program ( SSAC , PJ011059 to K.H.N.), Rural Development Administration , Republic of Korea, and the SRC Research Center for Women's Diseases of Sookmyung Women's University (2011) (grant # 3-1103-0020 to K.H.N.), and by the National Research Foundation ( NRF-2012R1A2A2A01009953 to M.M.L.), and in part by project IBS-R013-G2-2014-a00 to J.M.K. Publisher Copyright: {\textcopyright} 2016 The Author.",

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AU - Kwak, June M.

AU - Nam, Kyoung Hee

N1 - Funding Information: This work was supported by grants from the Next-Generation BioGreen 21 Program ( SSAC , PJ011059 to K.H.N.), Rural Development Administration , Republic of Korea, and the SRC Research Center for Women's Diseases of Sookmyung Women's University (2011) (grant # 3-1103-0020 to K.H.N.), and by the National Research Foundation ( NRF-2012R1A2A2A01009953 to M.M.L.), and in part by project IBS-R013-G2-2014-a00 to J.M.K. Publisher Copyright: © 2016 The Author.

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N2 - Stomatal movements are critical in regulating gas exchange for photosynthesis and water balance between plant tissues and the atmosphere. The plant hormone abscisic acid (ABA) plays key roles in regulating stomatal closure under various abiotic stresses. In this study, we revealed a novel role of BAK1 in guard cell ABA signaling. We found that the brassinosteroid (BR) signaling mutant bak1 lost more water than wild-type plants and showed ABA insensitivity in stomatal closure. ABA-induced OST1 expression and reactive oxygen species (ROS) production were also impaired in bak1. Unlike direct treatment with H2O2, overexpression of OST1 did not completely rescue the insensitivity of bak1 to ABA. We demonstrated that BAK1 forms a complex with OST1 near the plasma membrane and that the BAK1/OST1 complex is increased in response to ABA in planta. Brassinolide, the most active BR, exerted a negative effect on ABA-induced formation of the BAK1/OST1 complex and OST1 expression. Moreover, we found that BAK1 and ABI1 oppositely regulate OST1 phosphorylation in vitro, and that ABI1 interacts with BAK1 and inhibits the interaction of BAK1 and OST1. Taken together, our results suggest that BAK1 regulates ABA-induced stomatal closure in guard cells.

AB - Stomatal movements are critical in regulating gas exchange for photosynthesis and water balance between plant tissues and the atmosphere. The plant hormone abscisic acid (ABA) plays key roles in regulating stomatal closure under various abiotic stresses. In this study, we revealed a novel role of BAK1 in guard cell ABA signaling. We found that the brassinosteroid (BR) signaling mutant bak1 lost more water than wild-type plants and showed ABA insensitivity in stomatal closure. ABA-induced OST1 expression and reactive oxygen species (ROS) production were also impaired in bak1. Unlike direct treatment with H2O2, overexpression of OST1 did not completely rescue the insensitivity of bak1 to ABA. We demonstrated that BAK1 forms a complex with OST1 near the plasma membrane and that the BAK1/OST1 complex is increased in response to ABA in planta. Brassinolide, the most active BR, exerted a negative effect on ABA-induced formation of the BAK1/OST1 complex and OST1 expression. Moreover, we found that BAK1 and ABI1 oppositely regulate OST1 phosphorylation in vitro, and that ABI1 interacts with BAK1 and inhibits the interaction of BAK1 and OST1. Taken together, our results suggest that BAK1 regulates ABA-induced stomatal closure in guard cells.

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BRI1-Associated Receptor Kinase 1 Regulates Guard Cell ABA Signaling Mediated by Open Stomata 1 in Arabidopsis

Abstract

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