Broad-spectrum antiviral effect of Agrimonia pilosa extract on influenza viruses

Woo Jin Shin, Kwang Hee Lee, Myung Hwan Park, Baik Lin Seong

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Influenza virus continues to emerge and re-emerge, posing new threats for humans. Here we tested various Korean medicinal plant extracts for potential antiviral activity against influenza viruses. Among them, an extract of Agrimonia pilosa was shown to be highly effective against all three subtypes of human influenza viruses including H1N1 and H3N2 influenza A subtypes and influenza B virus. The EC50 value against influenza A virus, as tested by the plaque reduction assay on MDCK cells, was 14-23 μg/ml. The extract also exhibited a virucidal effect at a concentration of 160-570 ng/ml against influenzaAandBviruseswhen the viruseswere treatedwith the extract prior to plaque assay. Inaddition, when tested in embryonated chicken eggs the extract exhibited a strong inhibitory effect in ovo on the H9N2 avian influenza virus at a concentration of 280 ng/ml. Quantitative RT-PCR analysis data showed that the extract, to some degree, suppressed viral RNA synthesis in MDCK cells. HI and inhibition of neuraminidase were observed only at high concentrations of the extract. And yet, the extract's antiviral activity required direct contact between it and the virus, suggesting that its antiviral action is mediated by the viral membrane, but does not involve the two major surface antigens,HAandNA, of the virus. The broad-spectrum antiviral activity of Agrimonia pilosa extract on various subtypes of influenza viruses merits further investigation as it may provide a means of managing avian influenza infections in poultry farms and potential avian-human transmission.

Original languageEnglish
Pages (from-to)11-19
Number of pages9
JournalMicrobiology and Immunology
Volume54
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Agrimonia
Orthomyxoviridae
Antiviral Agents
Madin Darby Canine Kidney Cells
Influenza in Birds
Human Influenza
Viruses
Influenza B virus
Influenza A virus
Plant Extracts
Viral RNA
Neuraminidase
Poultry
Surface Antigens
Medicinal Plants
Eggs
Chickens
Polymerase Chain Reaction
Membranes
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Shin, Woo Jin ; Lee, Kwang Hee ; Park, Myung Hwan ; Seong, Baik Lin. / Broad-spectrum antiviral effect of Agrimonia pilosa extract on influenza viruses. In: Microbiology and Immunology. 2010 ; Vol. 54, No. 1. pp. 11-19.
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Broad-spectrum antiviral effect of Agrimonia pilosa extract on influenza viruses. / Shin, Woo Jin; Lee, Kwang Hee; Park, Myung Hwan; Seong, Baik Lin.

In: Microbiology and Immunology, Vol. 54, No. 1, 01.01.2010, p. 11-19.

Research output: Contribution to journalArticle

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