Broth microdilution method to detect extended-spectrum β-lactamases and AmpC β-lactamases in Enterobacteriaceae isolates by use of clavulanic acid and boronic acid as inhibitors

Hoon Jeong Seok, Wonkeun Song, Jae Seok Kim, Han Sung Kim, Man Lee Kyu

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14 Citations (Scopus)

Abstract

This study was designed to evaluate the performance of the broth microdilution (BMD) method to detect production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Enterobacteriaceae by using clavulanic acid (CA) and boronic acid (BA) as ESBL and AmpC β-lactamase inhibitors, respectively. A total of 100 clinical isolates of Enterobacteriaceae were analyzed. Mueller-Hinton broth containing serial twofold dilutions of cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), or cefepime (FEP) with or without either or both CA and BA was prepared. An eightfold or greater decrease in the MIC of CTX, CAZ, ATM, or FEP in the presence of CA and BA was considered a positive result for ESBL and plasmid-mediated AmpC β-lactamase (PABL), respectively. In tests with CA, expanded-spectrum β-lactams containing BA (CTX-BA, CAZ-BA, ATM-BA, and FEP-BA) showed higher positive rates in detecting ESBL producers than those without BA. The combination of CTX- and CAZ-based BMD tests with CA and BA showed sensitivity and specificity of 100% for the detection of ESBLs and PABLs. The BMD testing could be applicable for routine use in commercially available semiautomated systems for the detection of ESBLs and PABLs in Enterobacteriaceae.

Original languageEnglish
Pages (from-to)3409-3412
Number of pages4
JournalJournal of Clinical Microbiology
Volume47
Issue number11
DOIs
Publication statusPublished - 2009 Nov

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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