Byr4p, a Possible Regulator of Mitosis and Cytokinesis in Fission Yeast, Localizes to the Spindle Pole Body by its C-Terminal Domains

Miri Jwa, Sejeong Shin, Charles F. Albright, Kiwon Song

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume32
Issue number1
Publication statusPublished - 1999 Jan 31

Fingerprint

Spindle Pole Bodies
Cytokinesis
Schizosaccharomyces
Mitosis
Yeast
Poles
Proteins
Cell Cycle
Fusion reactions
Genes
Fluorescence
Cells
Microtubule-Organizing Center
Cell Nucleus Division
Anaphase
Essential Genes
Interphase
Open Reading Frames
Saccharomyces cerevisiae
Yeasts

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

@article{27d0c57473154f34b9f84367ef31c3f1,
title = "Byr4p, a Possible Regulator of Mitosis and Cytokinesis in Fission Yeast, Localizes to the Spindle Pole Body by its C-Terminal Domains",
abstract = "Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.",
author = "Miri Jwa and Sejeong Shin and Albright, {Charles F.} and Kiwon Song",
year = "1999",
month = "1",
day = "31",
language = "English",
volume = "32",
pages = "92--97",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "1",

}

Byr4p, a Possible Regulator of Mitosis and Cytokinesis in Fission Yeast, Localizes to the Spindle Pole Body by its C-Terminal Domains. / Jwa, Miri; Shin, Sejeong; Albright, Charles F.; Song, Kiwon.

In: Journal of Biochemistry and Molecular Biology, Vol. 32, No. 1, 31.01.1999, p. 92-97.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Byr4p, a Possible Regulator of Mitosis and Cytokinesis in Fission Yeast, Localizes to the Spindle Pole Body by its C-Terminal Domains

AU - Jwa, Miri

AU - Shin, Sejeong

AU - Albright, Charles F.

AU - Song, Kiwon

PY - 1999/1/31

Y1 - 1999/1/31

N2 - Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

AB - Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

UR - http://www.scopus.com/inward/record.url?scp=0033480717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033480717&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0033480717

VL - 32

SP - 92

EP - 97

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 1

ER -