C-reactive protein induces p53-mediated cell cycle arrest in H9c2 cardiac myocytes

Ji Won Choi, Kyung Hye Lee, Soo Hyuk Kim, Taewon Jin, Beom Seob Lee, Jaewon Oh, Ho Yeon Won, Soo Young Kim, Seok Min Kang, Ji Hyung Chung

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

C-reactive protein (CRP) is one of the most important biomarker for cardiovascular diseases. Recent studies have shown that CRP affects cell survival, differentiation and apoptosis. However, the effect of CRP on the cell cycle has not been studied yet. We investigated the cell cycle alterations and cellular mechanisms induced by CRP in H9c2 cardiac myocytes. Flow cytometry analysis showed that CRP-treated H9c2 cells displayed cell cycle arrest in G0/G1 phase. CRP treatment resulted in a significant reduction in the levels of CDK4, CDK6 and cyclin D1 in a concentration-dependent manner. Interestingly, CRP caused an increase in the p53 accumulation and its phosphorylation on Ser15, leading to induce p21 upregulation. Treatment with a specific p53 inhibitor, PFT-α restored the levels of CDK4 and CDK6. A significant increase of ERK1/2 phosphorylation level was detected in CRP-treated cells. Furthermore, pretreatment of a specific ERK inhibitor resulted in decreased p53 phosphorylation and p21 induction. ERK inhibitor pretreatment induced significant restoration of protein levels of CDK4 and CDK6, leading to re-entry into the cell cycle. In addition, increased phosphorylation of p53 and ERK induced by CRP was considerably reversed by Fc gamma receptor IIIa (FcγRIIIa) knock-down using siRNA. FcγRIIIa siRNA transfection also restored the levels of cell cycle proteins. Our study has provided the first proposal on the novel insights into how CRP directly affects cell cycle in cells.

Original languageEnglish
Pages (from-to)525-530
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume410
Issue number3
DOIs
Publication statusPublished - 2011 Jul 8

Fingerprint

Cell Cycle Checkpoints
Cardiac Myocytes
C-Reactive Protein
Cells
Phosphorylation
Cell Cycle
IgG Receptors
Small Interfering RNA
Cell Cycle Resting Phase
Cell Cycle Proteins
Flow cytometry
Reentry
Cyclin D1
G1 Phase
Biomarkers
Restoration
Transfection
Cell Differentiation
Cell Survival
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Choi, Ji Won ; Lee, Kyung Hye ; Kim, Soo Hyuk ; Jin, Taewon ; Lee, Beom Seob ; Oh, Jaewon ; Won, Ho Yeon ; Kim, Soo Young ; Kang, Seok Min ; Chung, Ji Hyung. / C-reactive protein induces p53-mediated cell cycle arrest in H9c2 cardiac myocytes. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 410, No. 3. pp. 525-530.
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C-reactive protein induces p53-mediated cell cycle arrest in H9c2 cardiac myocytes. / Choi, Ji Won; Lee, Kyung Hye; Kim, Soo Hyuk; Jin, Taewon; Lee, Beom Seob; Oh, Jaewon; Won, Ho Yeon; Kim, Soo Young; Kang, Seok Min; Chung, Ji Hyung.

In: Biochemical and Biophysical Research Communications, Vol. 410, No. 3, 08.07.2011, p. 525-530.

Research output: Contribution to journalArticle

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AU - Lee, Kyung Hye

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AU - Jin, Taewon

AU - Lee, Beom Seob

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AU - Chung, Ji Hyung

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