Caenorhabditis elegans as a screening tool for the endothelial cell-derived putative aging-related proteins detected by proteomic analysis

Moon Kyung Ha, Jeong Soo Cho, Ok Ryun Baik, Kwanghoon Lee, Hyeon-Sook Koo, Kee Yang Chung

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Endothelial cells go through progressive pathophysiologic modification as cellular senescence progresses. In vitro, endothelial cell senescence is accompanied by failure of proliferation and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in the organism in vivo and free radical theory is accepted to be the most plausible explanation for this process. We have screened proteins involved in both cellular senescence and reactive oxygen species induced condition using 2-D gel analysis and found that ubiquitin carboxyl terminal hydrolase L1, peroxyredoxin 2, peroxyredoxin 4, fatty acid binding proteins (FABPs), and 5'-AMP-activated protein kinase beta-1 subunit were candidate aging-related proteins. To evaluate in vivo function of these proteins, Caenorhabditis elegans (C. elegans) knock-down system using RNA interference was applied. Aging-specific expression of lipofucsin and the lifespan of knocked-down C. elegans were observed to assess the outcome. Interestingly, the inhibition of the genes led to short lifespan and earlier accumulation of lipofucsin with increasing age when compared with the wild type. These results suggest that the above genes may be related to cellular senescence process in determining the longevity in C. elegans and that gene inactivation renders animals susceptible to oxidative stress.

Original languageEnglish
Pages (from-to)3339-3351
Number of pages13
JournalProteomics
Volume6
Issue number11
DOIs
Publication statusPublished - 2006 Jun 1

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Cell Aging
Endothelial cells
Caenorhabditis elegans
Proteomics
Screening
Endothelial Cells
Genes
Aging of materials
Caenorhabditis elegans Proteins
AMP-Activated Protein Kinases
Fatty Acid-Binding Proteins
Proteins
Oxidative stress
Hydrolases
Ubiquitin
Free Radicals
Reactive Oxygen Species
Animals
Gels
RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

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title = "Caenorhabditis elegans as a screening tool for the endothelial cell-derived putative aging-related proteins detected by proteomic analysis",
abstract = "Endothelial cells go through progressive pathophysiologic modification as cellular senescence progresses. In vitro, endothelial cell senescence is accompanied by failure of proliferation and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in the organism in vivo and free radical theory is accepted to be the most plausible explanation for this process. We have screened proteins involved in both cellular senescence and reactive oxygen species induced condition using 2-D gel analysis and found that ubiquitin carboxyl terminal hydrolase L1, peroxyredoxin 2, peroxyredoxin 4, fatty acid binding proteins (FABPs), and 5'-AMP-activated protein kinase beta-1 subunit were candidate aging-related proteins. To evaluate in vivo function of these proteins, Caenorhabditis elegans (C. elegans) knock-down system using RNA interference was applied. Aging-specific expression of lipofucsin and the lifespan of knocked-down C. elegans were observed to assess the outcome. Interestingly, the inhibition of the genes led to short lifespan and earlier accumulation of lipofucsin with increasing age when compared with the wild type. These results suggest that the above genes may be related to cellular senescence process in determining the longevity in C. elegans and that gene inactivation renders animals susceptible to oxidative stress.",
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Caenorhabditis elegans as a screening tool for the endothelial cell-derived putative aging-related proteins detected by proteomic analysis. / Ha, Moon Kyung; Cho, Jeong Soo; Baik, Ok Ryun; Lee, Kwanghoon; Koo, Hyeon-Sook; Chung, Kee Yang.

In: Proteomics, Vol. 6, No. 11, 01.06.2006, p. 3339-3351.

Research output: Contribution to journalArticle

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AU - Koo, Hyeon-Sook

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