Caenorhabditis elegans germ cell proliferation and development were severely damaged in second generation dna-2 homozygotes. Even in the first generation, a much higher incidence of aberrant chromosomes in oocytes and resultantly higher embryonic lethality were found vs. wild type, when DNA breaks were induced by γ-rays or camptothecin. The deficiency of dna-2 in combination with RNA interference on mre-11 gene expression synergistically aggravated germ-line development, especially oocyte formation. These results suggest that C. elegans Dna-2 is involved in a DNA repair pathway paralleling homologous recombination or non-homologous end joining with mre-11 participation.
Bibliographical noteFunding Information:
We thank Dr. Yuji Kohara (National Institute of Genetics, Japan) for sending us EST clones. The N2, sqt-1(sc13), and sqt-1(e1350) C. elegans strains were obtained from the C. elegans Genetics Center (St. Paul, MN, USA), which is supported by the National Center for Research Resources. This work was supported by KOSEF Grant 20016-210-02-2 to J.A. and the Brain Korea 21 Project in 2003 to H.-S.K.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology