Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation

Jae Young Kwon, Jin Mo Park, Byung Soo Gim, Sang Jun Han, Junho Lee, Young Joon Kim

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Mediator proteins are required for transcriptional regulation of most genes in yeast. Mammalian Mediator homologs also function as transcriptional coactivators in vitro; however, their physiological role in gene-specific transcription is not yet known. To determine the role of Mediator proteins in the development of complex organisms, we purified putative Mediator complexes from Caenorhabditis elegans and analyzed their phenotypes in vivo. C. elegans Mediator homologs were assembled into two multiprotein complexes. RNA interference assays showed that the CeMed6, CeMed7. and CeMed10/CeNut2 gene products are required for the expression of developmentally regulated genes, but are dispensable for expression of the ubiquitously expressed genes tested in this study. Therefore, the gene-specific function of Mediator as an integrator of transcriptional regulatory signals is evolutionarily conserved and is essential for C. elegans development.

Original languageEnglish
Pages (from-to)14990-14995
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number26
DOIs
Publication statusPublished - 1999 Dec 21

All Science Journal Classification (ASJC) codes

  • General

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