CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion

Yun Hui Choi, Jing Lai, Myeong A. Kim, Aeryun Kim, Jinmoon Kim, Hanfu Su, Linhu Ge, Jeong Heon Cha

Research output: Contribution to journalArticlepeer-review

Abstract

Helicobacter pylori colonizes human gastric mucosa. Its infection is associated with gastric diseases including gastric cancer. CagA is one of the most important toxins produced by H. pylori. It is related to gastric cancer which can be injected into host cells via a type IV secretion system (T4SS). CagL is a structural component of T4SS apparatus, which triggers host cell signaling pathway. It has been reported that CagL polymorphisms may influence the severity of disease development. To explore the contribution of CagL polymorphisms between East Asian and Western H. pylori in pathogenesis, cagL gene in G27 H. pylori was swapped by K74 cagL which is identical to East Asian CagL consensus sequence and by Western 26695 H. pylori, resulting in G27 ΔcagL/cagLK74 and G27 ΔcagL/cagL26695, respectively. Intriguingly, G27 ΔcagL/cagLK74 showed significantly less ability of IL-8 induction than G27 ΔcagL/cagL26695 while displayed similar abilities of CagA phosphorylation, and cell elongation. Taken together, this study suggests that the CagL polymorphism may influence IL-8 induction, and K74 CagL has less ability to induce IL-8 secretion than G27 or 26695 CagL. Further research should address how the different capabilities of IL-8 induction between intraspecies-CagL are associated with the large differences of the incidence of gastric cancer between East Asian and Western countries.

Original languageEnglish
Pages (from-to)763-770
Number of pages8
JournalJournal of Microbiology
Volume59
Issue number8
DOIs
Publication statusPublished - 2021 Aug

Bibliographical note

Funding Information:
This work was supported by the Korean College of Helicobacter and Upper Gastrointestinal Research Foundation Grant KCHUGR-202001005.

Publisher Copyright:
© 2021, The Microbiological Society of Korea.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion'. Together they form a unique fingerprint.

Cite this