Abstract
Calcineurin is a Ca2+-calmodulin-dependent serine/threonine protein phosphatase that has been implicated in various signaling pathways. Here we report the identification and characterization of calcineurin genes in Caenorhabditis elegans (cna-1 and cnb-1), which share high homology with Drosophila and mammalian calcineurin genes. C. elegans calcineurin binds calcium and functions as a heterodimeric protein phosphatase establishing its biochemical conservation in the nematode. Calcineurin is expressed in hypodermal seam cells, body-wall muscle, vulva muscle, neuronal cells, and in sperm and the spermatheca. cnb-1 mutants showed pleiotropic defects including lethargic movement and delayed egg-laying. Interestingly, these characteristic defects resembled phenotypes observed in gain-of-function mutants of unc-43/Ca2+-calmodulin-dependent protein kinase II (CaMKII) and goa-1/Go-protein α-subunit. Double mutants of cnb-1 and unc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that caldneurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.
| Original language | English |
|---|---|
| Pages (from-to) | 3281-3293 |
| Number of pages | 13 |
| Journal | Molecular Biology of the Cell |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2002 Sep 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
Cite this
}
Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans. / Bandyopadhyay, Jaya; Lee, Jiyeon; Lee, Jungsoo; Lee, Jin Il; Yu, Jae Ran; Jee, Changhoon; Cho, Jeong Hoon; Jung, Sunki; Lee, Myon Hee; Zannoni, Sonia; Singson, Andrew; Kim, Do Han; Koo, Hyeon Sook; Ahnn, Joohong.
In: Molecular Biology of the Cell, Vol. 13, No. 9, 01.09.2002, p. 3281-3293.Research output: Contribution to journal › Article
TY - JOUR
T1 - Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans
AU - Bandyopadhyay, Jaya
AU - Lee, Jiyeon
AU - Lee, Jungsoo
AU - Lee, Jin Il
AU - Yu, Jae Ran
AU - Jee, Changhoon
AU - Cho, Jeong Hoon
AU - Jung, Sunki
AU - Lee, Myon Hee
AU - Zannoni, Sonia
AU - Singson, Andrew
AU - Kim, Do Han
AU - Koo, Hyeon Sook
AU - Ahnn, Joohong
PY - 2002/9/1
Y1 - 2002/9/1
N2 - Calcineurin is a Ca2+-calmodulin-dependent serine/threonine protein phosphatase that has been implicated in various signaling pathways. Here we report the identification and characterization of calcineurin genes in Caenorhabditis elegans (cna-1 and cnb-1), which share high homology with Drosophila and mammalian calcineurin genes. C. elegans calcineurin binds calcium and functions as a heterodimeric protein phosphatase establishing its biochemical conservation in the nematode. Calcineurin is expressed in hypodermal seam cells, body-wall muscle, vulva muscle, neuronal cells, and in sperm and the spermatheca. cnb-1 mutants showed pleiotropic defects including lethargic movement and delayed egg-laying. Interestingly, these characteristic defects resembled phenotypes observed in gain-of-function mutants of unc-43/Ca2+-calmodulin-dependent protein kinase II (CaMKII) and goa-1/Go-protein α-subunit. Double mutants of cnb-1 and unc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that caldneurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.
AB - Calcineurin is a Ca2+-calmodulin-dependent serine/threonine protein phosphatase that has been implicated in various signaling pathways. Here we report the identification and characterization of calcineurin genes in Caenorhabditis elegans (cna-1 and cnb-1), which share high homology with Drosophila and mammalian calcineurin genes. C. elegans calcineurin binds calcium and functions as a heterodimeric protein phosphatase establishing its biochemical conservation in the nematode. Calcineurin is expressed in hypodermal seam cells, body-wall muscle, vulva muscle, neuronal cells, and in sperm and the spermatheca. cnb-1 mutants showed pleiotropic defects including lethargic movement and delayed egg-laying. Interestingly, these characteristic defects resembled phenotypes observed in gain-of-function mutants of unc-43/Ca2+-calmodulin-dependent protein kinase II (CaMKII) and goa-1/Go-protein α-subunit. Double mutants of cnb-1 and unc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that caldneurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.
UR - http://www.scopus.com/inward/record.url?scp=18544379785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18544379785&partnerID=8YFLogxK
U2 - 10.1091/mbc.E02-01-0005
DO - 10.1091/mbc.E02-01-0005
M3 - Article
C2 - 12221132
AN - SCOPUS:18544379785
VL - 13
SP - 3281
EP - 3293
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 9
ER -