Calmidazolium chloride inhibits growth of murine embryonal carcinoma cells, a model of cancer stem-like cells

Jina Lee, Min Seong Kim, Min Aeh Kim, Yeun Kyu Jang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Calmidazolium chloride (CMZ) is widely used as a calmodulin (CaM) antagonist, but is also known to induce apoptosis in certain cancer cell lines. However, in spite of the importance of cancer stem cells (CSCs) in cancer therapy, the effects of CMZ on CSCs are not yet well understood. We investigated the effects of CMZ on the F9 embryonal carcinoma cell (ECC) line as a surrogate model of CSCs. To avoid bias due to culture conditions, F9 ECCs and E14 embryonic stem cells (ESCs) were grown in the same culture medium. Results obtained using a cell-counting kit showed that CMZ significantly inhibited growth in F9 ECCs compared with growth in E14 ESCs. CMZ also induced apoptosis of F9 ECCs, but not of E14 ESCs, which was associated with caspase-3 activation and an increased fraction of the sub-G1 cell population. In addition, our data revealed that the expression of stemness-related genes including c-Myc was selectively down regulated in CMZ-treated F9 ECCs. Our results suggest that CMZ can inhibit the growth of ECCs by inducing apoptosis and down regulating stemness-related genes, without causing any harm to normal stem cells. These findings indicate a potential application of CMZ in the development of anti-CSC therapeutics.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalToxicology in Vitro
Volume35
DOIs
Publication statusPublished - 2016 Sep 1

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calmidazolium
Embryonal Carcinoma Stem Cells
Neoplastic Stem Cells
Stem cells
Cells
Growth
Embryonic Stem Cells
Apoptosis
Genes
Cell Line
myc Genes
Calmodulin
Cell culture
Caspase 3
Culture Media
Neoplasms
Stem Cells
Chemical activation

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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abstract = "Calmidazolium chloride (CMZ) is widely used as a calmodulin (CaM) antagonist, but is also known to induce apoptosis in certain cancer cell lines. However, in spite of the importance of cancer stem cells (CSCs) in cancer therapy, the effects of CMZ on CSCs are not yet well understood. We investigated the effects of CMZ on the F9 embryonal carcinoma cell (ECC) line as a surrogate model of CSCs. To avoid bias due to culture conditions, F9 ECCs and E14 embryonic stem cells (ESCs) were grown in the same culture medium. Results obtained using a cell-counting kit showed that CMZ significantly inhibited growth in F9 ECCs compared with growth in E14 ESCs. CMZ also induced apoptosis of F9 ECCs, but not of E14 ESCs, which was associated with caspase-3 activation and an increased fraction of the sub-G1 cell population. In addition, our data revealed that the expression of stemness-related genes including c-Myc was selectively down regulated in CMZ-treated F9 ECCs. Our results suggest that CMZ can inhibit the growth of ECCs by inducing apoptosis and down regulating stemness-related genes, without causing any harm to normal stem cells. These findings indicate a potential application of CMZ in the development of anti-CSC therapeutics.",
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Calmidazolium chloride inhibits growth of murine embryonal carcinoma cells, a model of cancer stem-like cells. / Lee, Jina; Kim, Min Seong; Kim, Min Aeh; Jang, Yeun Kyu.

In: Toxicology in Vitro, Vol. 35, 01.09.2016, p. 86-92.

Research output: Contribution to journalArticle

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