Calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells

Yun Soo Jang, Kyoung Ju Song, Ju Young Kim, Young Ah Lee, Kyeong Ah Kim, Sang Kyou Lee, Myeong Heon Shin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and μ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and μ-calpain may be involved in colon epithelial cell death induced by E. histolytica.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalKorean Journal of Parasitology
Volume49
Issue number2
DOIs
Publication statusPublished - 2011 Jun

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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