Calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells

Yun Soo Jang, Kyoung Ju Song, Ju Young Kim, Young Ah Lee, Kyeong Ah Kim, Sang Kyou Lee, Myeong Heon Shin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and μ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and μ-calpain may be involved in colon epithelial cell death induced by E. histolytica.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalKorean Journal of Parasitology
Volume49
Issue number2
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Entamoeba histolytica
Calpain
HT29 Cells
Epithelial Cells
Cell Death
Colon
Amoeba
Amoebic Dysentery
Amoebic Liver Abscess
Propidium
Small Interfering RNA
Parasites

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Jang, Yun Soo ; Song, Kyoung Ju ; Kim, Ju Young ; Lee, Young Ah ; Kim, Kyeong Ah ; Lee, Sang Kyou ; Shin, Myeong Heon. / Calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells. In: Korean Journal of Parasitology. 2011 ; Vol. 49, No. 2. pp. 177-180.
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Calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells. / Jang, Yun Soo; Song, Kyoung Ju; Kim, Ju Young; Lee, Young Ah; Kim, Kyeong Ah; Lee, Sang Kyou; Shin, Myeong Heon.

In: Korean Journal of Parasitology, Vol. 49, No. 2, 01.06.2011, p. 177-180.

Research output: Contribution to journalArticle

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