Abstract
Camptothecin is an anti-cancer drug extracted from Camptotheca acuminata, a tree native to mainland China. Phase III clinical trials for camptothecin have been completed, and it is now used as a chemotherapeutic reagent. We identified a novel function of camptothecin that affects adipocyte differentiation. Following treatment with camptothecin, endogenous or overexpressed PPARγ becomes destabilized; this was prevented in the presence of MG132, a proteasome inhibitor. Our findings suggest that camptothecin is able to induce proteasome-dependent degradation of PPARγ. The ubiquitylation of PPARγ increased in the presence of camptothecin. Adipogenic differentiation of 3T3-L1 cells was prevented by campothecin and topotecan, but not by irinotecan, confirming our initial findings. Our results suggest a possible role for camptothecin analogs in the regulation of PPARγ.
| Original language | English |
|---|---|
| Pages (from-to) | 1122-1128 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 463 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2015 Jul 13 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Camptothecin and topotecan inhibit adipocyte differentiation by inducing degradation of PPARγ. / Kim, Jung Hoon; Jeong, Manhyung; Lee, Sang Sik; Song, Jaewhan.
In: Biochemical and Biophysical Research Communications, Vol. 463, No. 4, 13.07.2015, p. 1122-1128.Research output: Contribution to journal › Article
TY - JOUR
T1 - Camptothecin and topotecan inhibit adipocyte differentiation by inducing degradation of PPARγ
AU - Kim, Jung Hoon
AU - Jeong, Manhyung
AU - Lee, Sang Sik
AU - Song, Jaewhan
PY - 2015/7/13
Y1 - 2015/7/13
N2 - Camptothecin is an anti-cancer drug extracted from Camptotheca acuminata, a tree native to mainland China. Phase III clinical trials for camptothecin have been completed, and it is now used as a chemotherapeutic reagent. We identified a novel function of camptothecin that affects adipocyte differentiation. Following treatment with camptothecin, endogenous or overexpressed PPARγ becomes destabilized; this was prevented in the presence of MG132, a proteasome inhibitor. Our findings suggest that camptothecin is able to induce proteasome-dependent degradation of PPARγ. The ubiquitylation of PPARγ increased in the presence of camptothecin. Adipogenic differentiation of 3T3-L1 cells was prevented by campothecin and topotecan, but not by irinotecan, confirming our initial findings. Our results suggest a possible role for camptothecin analogs in the regulation of PPARγ.
AB - Camptothecin is an anti-cancer drug extracted from Camptotheca acuminata, a tree native to mainland China. Phase III clinical trials for camptothecin have been completed, and it is now used as a chemotherapeutic reagent. We identified a novel function of camptothecin that affects adipocyte differentiation. Following treatment with camptothecin, endogenous or overexpressed PPARγ becomes destabilized; this was prevented in the presence of MG132, a proteasome inhibitor. Our findings suggest that camptothecin is able to induce proteasome-dependent degradation of PPARγ. The ubiquitylation of PPARγ increased in the presence of camptothecin. Adipogenic differentiation of 3T3-L1 cells was prevented by campothecin and topotecan, but not by irinotecan, confirming our initial findings. Our results suggest a possible role for camptothecin analogs in the regulation of PPARγ.
UR - http://www.scopus.com/inward/record.url?scp=84936985919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84936985919&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2015.06.069
DO - 10.1016/j.bbrc.2015.06.069
M3 - Article
C2 - 26079886
AN - SCOPUS:84936985919
VL - 463
SP - 1122
EP - 1128
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -