Can blood components with age-related changes influence the ageing of endothelial cells?

Ji Yeon Noh, SangHo Oh, JuHee Lee, Yeon Sook Kwon, Dong Jin Ryu, Kwanghoon Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Research on vascular endothelial cell ageing helps elucidate the pathogenesis of diseases associated with cell ageing. To investigate endothelial senescence, we used 2-DE coupled with MS to perform a proteomic analysis of: (i) peripheral blood mononuclear cells (PBMCs) from donors in their 20 s ('young') or 60 s ('old') and (ii) human dermal microvascular endothelial cells (HDMECs) treated with sera from young and old donors. Identified proteins could be classified into several functional categories: (i) cytoskeletal regulators: CapG and cofilin 1; (ii) stress response and signal pathway proteins: TXNDC5 and RSU-1; and (iii) apoptosis proteins: Annexin V. We confirmed by Western blot a decrease of RSU-1, CapG and TXNDC5 in PBMCs from old donors. RSU-1, which regulates signal transduction of the downstream Ras, showed decreased mRNA and protein levels in PBMCs from old donors and decreased mRNA levels in HDMECs treated with sera from old donors. In addition, Ras protein levels were increased in PBMCs from old donors. These data indicate that reduced RSU-1 might induce Ras expression, which subsequently could provoke Ras-induced senescence. In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing.

Original languageEnglish
Pages (from-to)339-346
Number of pages8
JournalExperimental Dermatology
Volume19
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Endothelial cells
Cell Aging
Blood Cells
Blood
Endothelial Cells
Aging of materials
Cofilin 1
Signal Transduction
Proteins
ras Proteins
Messenger RNA
Skin
Cytoskeletal Proteins
Annexin A5
Heat-Shock Proteins
Serum
Signal transduction
Proteomics
Western Blotting
Apoptosis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

@article{b2a72322cc0941feba01a9da7ce8a1c4,
title = "Can blood components with age-related changes influence the ageing of endothelial cells?",
abstract = "Research on vascular endothelial cell ageing helps elucidate the pathogenesis of diseases associated with cell ageing. To investigate endothelial senescence, we used 2-DE coupled with MS to perform a proteomic analysis of: (i) peripheral blood mononuclear cells (PBMCs) from donors in their 20 s ('young') or 60 s ('old') and (ii) human dermal microvascular endothelial cells (HDMECs) treated with sera from young and old donors. Identified proteins could be classified into several functional categories: (i) cytoskeletal regulators: CapG and cofilin 1; (ii) stress response and signal pathway proteins: TXNDC5 and RSU-1; and (iii) apoptosis proteins: Annexin V. We confirmed by Western blot a decrease of RSU-1, CapG and TXNDC5 in PBMCs from old donors. RSU-1, which regulates signal transduction of the downstream Ras, showed decreased mRNA and protein levels in PBMCs from old donors and decreased mRNA levels in HDMECs treated with sera from old donors. In addition, Ras protein levels were increased in PBMCs from old donors. These data indicate that reduced RSU-1 might induce Ras expression, which subsequently could provoke Ras-induced senescence. In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing.",
author = "Noh, {Ji Yeon} and SangHo Oh and JuHee Lee and Kwon, {Yeon Sook} and Ryu, {Dong Jin} and Kwanghoon Lee",
year = "2010",
month = "4",
day = "1",
doi = "10.1111/j.1600-0625.2009.01010.x",
language = "English",
volume = "19",
pages = "339--346",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "4",

}

Can blood components with age-related changes influence the ageing of endothelial cells? / Noh, Ji Yeon; Oh, SangHo; Lee, JuHee; Kwon, Yeon Sook; Ryu, Dong Jin; Lee, Kwanghoon.

In: Experimental Dermatology, Vol. 19, No. 4, 01.04.2010, p. 339-346.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Can blood components with age-related changes influence the ageing of endothelial cells?

AU - Noh, Ji Yeon

AU - Oh, SangHo

AU - Lee, JuHee

AU - Kwon, Yeon Sook

AU - Ryu, Dong Jin

AU - Lee, Kwanghoon

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Research on vascular endothelial cell ageing helps elucidate the pathogenesis of diseases associated with cell ageing. To investigate endothelial senescence, we used 2-DE coupled with MS to perform a proteomic analysis of: (i) peripheral blood mononuclear cells (PBMCs) from donors in their 20 s ('young') or 60 s ('old') and (ii) human dermal microvascular endothelial cells (HDMECs) treated with sera from young and old donors. Identified proteins could be classified into several functional categories: (i) cytoskeletal regulators: CapG and cofilin 1; (ii) stress response and signal pathway proteins: TXNDC5 and RSU-1; and (iii) apoptosis proteins: Annexin V. We confirmed by Western blot a decrease of RSU-1, CapG and TXNDC5 in PBMCs from old donors. RSU-1, which regulates signal transduction of the downstream Ras, showed decreased mRNA and protein levels in PBMCs from old donors and decreased mRNA levels in HDMECs treated with sera from old donors. In addition, Ras protein levels were increased in PBMCs from old donors. These data indicate that reduced RSU-1 might induce Ras expression, which subsequently could provoke Ras-induced senescence. In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing.

AB - Research on vascular endothelial cell ageing helps elucidate the pathogenesis of diseases associated with cell ageing. To investigate endothelial senescence, we used 2-DE coupled with MS to perform a proteomic analysis of: (i) peripheral blood mononuclear cells (PBMCs) from donors in their 20 s ('young') or 60 s ('old') and (ii) human dermal microvascular endothelial cells (HDMECs) treated with sera from young and old donors. Identified proteins could be classified into several functional categories: (i) cytoskeletal regulators: CapG and cofilin 1; (ii) stress response and signal pathway proteins: TXNDC5 and RSU-1; and (iii) apoptosis proteins: Annexin V. We confirmed by Western blot a decrease of RSU-1, CapG and TXNDC5 in PBMCs from old donors. RSU-1, which regulates signal transduction of the downstream Ras, showed decreased mRNA and protein levels in PBMCs from old donors and decreased mRNA levels in HDMECs treated with sera from old donors. In addition, Ras protein levels were increased in PBMCs from old donors. These data indicate that reduced RSU-1 might induce Ras expression, which subsequently could provoke Ras-induced senescence. In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing.

UR - http://www.scopus.com/inward/record.url?scp=77951711649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951711649&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0625.2009.01010.x

DO - 10.1111/j.1600-0625.2009.01010.x

M3 - Article

VL - 19

SP - 339

EP - 346

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 4

ER -