CAND1 enhances deneddylation of CUL1 by COP9 signalosome

Kyoeng Woo Min, Mun Jin Kwon, Hyoung Seo Park, Yoon Park, Kim Yoon Sungjoo, Jong-Bok Yoon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cullin-RING ligases (CRLs) regulate diverse cellular functions such as cell cycle progression and cytokine signaling by ubiquitinating key regulatory proteins. The activity of CRLs is controlled by Nedd8 modification of the cullin subunits. Recent reports have suggested that CAND1, which specifically binds to unmodified CUL1 but not to neddylated one, is required for the in vivo function of SCFs, the CUL1-containing CRLs. We show here that CAND1 and COP9 signalosome (CSN), the major deneddylase of cullins, bind to unneddylated CUL1 in a mutually exclusive way. The suppression of CAND1 expression by small inhibitory RNA enhanced the interaction between CUL1 and CSN, suggesting that CAND1 inhibited the binding of CSN to CUL1. We found that the binding of CSN to CUL1 required the four helix bundle in CUL1 C-terminal domain, which was wrapped around by CAND1 in the CAND1-CUL1-Rbx1 complex. CAND1 greatly facilitated CSN-mediated deneddylation of CUL1 in vitro, which was dependent on its binding to CUL1. Our data suggest that enhancement of CSN-mediated deneddylation by CAND1 may contribute to its function as a positive regulator of SCFs in vivo.

Original languageEnglish
Pages (from-to)867-874
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number3
DOIs
Publication statusPublished - 2005 Sep 2

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Cullin Proteins
Ligases
COP9 signalosome complex
Cell Cycle
Cells
RNA
Cytokines
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Min, Kyoeng Woo ; Kwon, Mun Jin ; Park, Hyoung Seo ; Park, Yoon ; Sungjoo, Kim Yoon ; Yoon, Jong-Bok. / CAND1 enhances deneddylation of CUL1 by COP9 signalosome. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 334, No. 3. pp. 867-874.
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CAND1 enhances deneddylation of CUL1 by COP9 signalosome. / Min, Kyoeng Woo; Kwon, Mun Jin; Park, Hyoung Seo; Park, Yoon; Sungjoo, Kim Yoon; Yoon, Jong-Bok.

In: Biochemical and Biophysical Research Communications, Vol. 334, No. 3, 02.09.2005, p. 867-874.

Research output: Contribution to journalArticle

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