CAP modifies the structure of a model protein from thermophilic bacteria: Mechanisms of CAP-mediated inactivation

Pankaj Attri, Jeongmin Han, Sooho Choi, Eun Ha Choi, Annemie Bogaerts, Weontae Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.

Original languageEnglish
Article number10218
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Plasma Gases
Bacteria
Methanobacterium
Proteins
Food Industry
Fluorescence Spectrometry
Molecular Dynamics Simulation
Circular Dichroism
Magnetic Resonance Spectroscopy
Hot Temperature
Air

All Science Journal Classification (ASJC) codes

  • General

Cite this

Attri, Pankaj ; Han, Jeongmin ; Choi, Sooho ; Choi, Eun Ha ; Bogaerts, Annemie ; Lee, Weontae. / CAP modifies the structure of a model protein from thermophilic bacteria : Mechanisms of CAP-mediated inactivation. In: Scientific reports. 2018 ; Vol. 8, No. 1.
@article{caded6665c3248e0b913af2163ac992f,
title = "CAP modifies the structure of a model protein from thermophilic bacteria: Mechanisms of CAP-mediated inactivation",
abstract = "Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.",
author = "Pankaj Attri and Jeongmin Han and Sooho Choi and Choi, {Eun Ha} and Annemie Bogaerts and Weontae Lee",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-28600-w",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

CAP modifies the structure of a model protein from thermophilic bacteria : Mechanisms of CAP-mediated inactivation. / Attri, Pankaj; Han, Jeongmin; Choi, Sooho; Choi, Eun Ha; Bogaerts, Annemie; Lee, Weontae.

In: Scientific reports, Vol. 8, No. 1, 10218, 01.12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CAP modifies the structure of a model protein from thermophilic bacteria

T2 - Mechanisms of CAP-mediated inactivation

AU - Attri, Pankaj

AU - Han, Jeongmin

AU - Choi, Sooho

AU - Choi, Eun Ha

AU - Bogaerts, Annemie

AU - Lee, Weontae

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.

AB - Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.

UR - http://www.scopus.com/inward/record.url?scp=85049637234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049637234&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-28600-w

DO - 10.1038/s41598-018-28600-w

M3 - Article

C2 - 29977069

AN - SCOPUS:85049637234

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10218

ER -