Capacitance-based assay for real-time monitoring of endocytosis and cell viability

Rimi Lee, Jihun Kim, Sook Young Kim, Seon Mi Jang, Sun Mi Lee, In Hong Choi, Seung Woo Park, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.

Original languageEnglish
Pages (from-to)2377-2384
Number of pages8
JournalLab on a chip
Volume12
Issue number13
DOIs
Publication statusPublished - 2012 Jul 7

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Lee, R., Kim, J., Kim, S. Y., Jang, S. M., Lee, S. M., Choi, I. H., Park, S. W., Shin, J. S., & Yoo, K. H. (2012). Capacitance-based assay for real-time monitoring of endocytosis and cell viability. Lab on a chip, 12(13), 2377-2384. https://doi.org/10.1039/c2lc21236f