Capacitance-based real time monitoring of receptor-mediated endocytosis

Rimi Lee, Pyung Hwan Kim, Joung Woo Choi, Kwon Oh-Joon, Kyujung Kim, Donghyun Kim, Chae Ok Yun, Kyung-hwa Yoo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. In this study, we developed a capacitance sensor to monitor receptor-mediated endocytosis in real time. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination between receptor-mediated endocytosis and non-specific pinocytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening. We showed that the capacitance sensor array could rapidly identify antibodies or ligands with high specificity for target molecules. We propose that the capacitance sensor array will provide a valuable tool for high-throughput screening.

Original languageEnglish
Pages (from-to)1325-1332
Number of pages8
JournalBiosensors and Bioelectronics
Volume25
Issue number6
DOIs
Publication statusPublished - 2010 Feb 15

Fingerprint

Endocytosis
Capacitance
Pinocytosis
Monitoring
Sensor arrays
Antibodies
Essential Genes
Adenoviridae
Nanoparticles
Ligands
Cell Line
Sensors
Screening
Throughput
Pharmaceutical Preparations
Drug delivery
Genes
Cells
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Lee, Rimi ; Kim, Pyung Hwan ; Choi, Joung Woo ; Oh-Joon, Kwon ; Kim, Kyujung ; Kim, Donghyun ; Yun, Chae Ok ; Yoo, Kyung-hwa. / Capacitance-based real time monitoring of receptor-mediated endocytosis. In: Biosensors and Bioelectronics. 2010 ; Vol. 25, No. 6. pp. 1325-1332.
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Capacitance-based real time monitoring of receptor-mediated endocytosis. / Lee, Rimi; Kim, Pyung Hwan; Choi, Joung Woo; Oh-Joon, Kwon; Kim, Kyujung; Kim, Donghyun; Yun, Chae Ok; Yoo, Kyung-hwa.

In: Biosensors and Bioelectronics, Vol. 25, No. 6, 15.02.2010, p. 1325-1332.

Research output: Contribution to journalArticle

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