Capacitive immunoaffinity biosensor by using diamond-like carbon (DLC) electrode

Ju Kyung Lee, Gun Ho Noh, Jae Chul Pyun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A new capacitive biosensor based on chronoamperometry was developed by using diamond-like carbon (DLC) film as an electric isolating layer. The electrochemical properties of the DLC layered electrode were analyzed by using a cyclic voltammetry. The immunoaffinity layer was prepared by physically adsorbing the anti-horseradish peroxidase (HRP) antibodies on the DLC electrode, and the feasibility of this capacitive biosensor was tested by using HRP as a model analyte. The limit of detection (LOD) was measured to be 0.01 pg/mL, and the detection range was determined to range from less than 0.1 pg/mL to more than 1 ng/mL. The applicability of the new capacitive biosensor was demonstrated by detecting C-reactive protein(CRP) which is known as a biomarker for the inflammatory diseases.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalBiochip Journal
Volume3
Issue number4
Publication statusPublished - 2009 Jan 1

Fingerprint

Diamond
Biosensing Techniques
Biosensors
Diamonds
Electrodes
Carbon
Horseradish Peroxidase
Diamond like carbon films
Chronoamperometry
Biomarkers
Electrochemical properties
Antibodies
C-Reactive Protein
Cyclic voltammetry
Limit of Detection
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

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Capacitive immunoaffinity biosensor by using diamond-like carbon (DLC) electrode. / Lee, Ju Kyung; Noh, Gun Ho; Pyun, Jae Chul.

In: Biochip Journal, Vol. 3, No. 4, 01.01.2009, p. 287-292.

Research output: Contribution to journalArticle

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