Capacity of dental pulp differentiation after tooth transplantation

Ryoichiro Ogawa, Chikara Saito, Hansung Jung, Hayato Ohshima

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Under pathological conditions, dental pulp elaborates both bone and dentin matrix in which the contribution of periodontal tissue cannot be excluded. This study has aimed to clarify the capability of dental pulp to deposit bone matrix in an auto-graft experiment by using (1) immunohistochemistry for 5-bromo-2′-deoxyuridine (BrdU) and nestin and (2) histochemistry for tartrate-resistant acid phosphatase (TRAP). Following the extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately transplanted into the sublingual region. On Days 5-7, tubular dentin formation commenced next to the pre-existing dentin at the pulp horn in which nestin-positive odontoblast-like cells were arranged. Up until Day 14, bone-like tissue formation occurred in the pulp chamber in which intense TRAP-positive cells appeared. These results suggest that odontoblast- and osteoblast-lineage cells reside in the dental pulp. Overall, specific dental pulp regeneration should provide fundamental knowledge for the realization of human tooth regeneration in the near future.

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalCell and Tissue Research
Volume326
Issue number3
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Dental Pulp
Tooth
Dentin
Transplantation
Odontoblasts
Nestin
Bone Matrix
Regeneration
Mouth Floor
Dental Pulp Cavity
Bromodeoxyuridine
Horns
Osteoblasts
Immunohistochemistry
Transplants
Bone and Bones
Tartrate-Resistant Acid Phosphatase

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Ogawa, Ryoichiro ; Saito, Chikara ; Jung, Hansung ; Ohshima, Hayato. / Capacity of dental pulp differentiation after tooth transplantation. In: Cell and Tissue Research. 2006 ; Vol. 326, No. 3. pp. 715-724.
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Capacity of dental pulp differentiation after tooth transplantation. / Ogawa, Ryoichiro; Saito, Chikara; Jung, Hansung; Ohshima, Hayato.

In: Cell and Tissue Research, Vol. 326, No. 3, 01.12.2006, p. 715-724.

Research output: Contribution to journalArticle

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