Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation

Yasuhiko Takamori, Hironobu Suzuki, Kuniko Nakakura-Ohshima, Jinglei Cai, Sung Won Cho, Hansung Jung, Hayato Ohshima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue.

Original languageEnglish
Pages (from-to)1075-1086
Number of pages12
JournalJournal of Histochemistry and Cytochemistry
Volume56
Issue number12
DOIs
Publication statusPublished - 2008 Dec 1

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Dental Pulp
Homologous Transplantation
Tooth
Dentin
Odontoblasts
Bone and Bones
Osteoblasts
Tooth Replantation
Mouth Floor
Nestin
Dental Pulp Cavity
Osteoclasts
Horns
Transgenic Mice
Transplantation
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Takamori, Yasuhiko ; Suzuki, Hironobu ; Nakakura-Ohshima, Kuniko ; Cai, Jinglei ; Cho, Sung Won ; Jung, Hansung ; Ohshima, Hayato. / Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation. In: Journal of Histochemistry and Cytochemistry. 2008 ; Vol. 56, No. 12. pp. 1075-1086.
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Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation. / Takamori, Yasuhiko; Suzuki, Hironobu; Nakakura-Ohshima, Kuniko; Cai, Jinglei; Cho, Sung Won; Jung, Hansung; Ohshima, Hayato.

In: Journal of Histochemistry and Cytochemistry, Vol. 56, No. 12, 01.12.2008, p. 1075-1086.

Research output: Contribution to journalArticle

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