Abstract
Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue.
| Original language | English |
|---|---|
| Pages (from-to) | 1075-1086 |
| Number of pages | 12 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 56 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Anatomy
- Histology
Cite this
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Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation. / Takamori, Yasuhiko; Suzuki, Hironobu; Nakakura-Ohshima, Kuniko; Cai, Jinglei; Cho, Sung Won; Jung, Han Sung; Ohshima, Hayato.
In: Journal of Histochemistry and Cytochemistry, Vol. 56, No. 12, 01.12.2008, p. 1075-1086.Research output: Contribution to journal › Article
TY - JOUR
T1 - Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation
AU - Takamori, Yasuhiko
AU - Suzuki, Hironobu
AU - Nakakura-Ohshima, Kuniko
AU - Cai, Jinglei
AU - Cho, Sung Won
AU - Jung, Han Sung
AU - Ohshima, Hayato
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue.
AB - Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue.
UR - http://www.scopus.com/inward/record.url?scp=56449123872&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56449123872&partnerID=8YFLogxK
U2 - 10.1369/jhc.2008.951558
DO - 10.1369/jhc.2008.951558
M3 - Article
C2 - 18765839
AN - SCOPUS:56449123872
VL - 56
SP - 1075
EP - 1086
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 12
ER -