Capillary-scale direct measurement of hemoglobin concentration of erythrocytes using photothermal angular light scattering

Uihan Kim, Jaewoo Song, Donghak Lee, Suho Ryu, Soocheol Kim, Jaehyun Hwang, Chulmin Joo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9. g/dL with a detection limit of ~0.12. g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (. r>0.96), and yielded a precision of 0.63. g/dL for a blood sample of 9.0. g/dL.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalBiosensors and Bioelectronics
Volume74
DOIs
Publication statusPublished - 2015 Dec 5

Fingerprint

Hemoglobin
Light scattering
Hemoglobins
Blood
Erythrocytes
Light
Refractometry
Refractive index
Hematology
Lighting
Iron oxides
Limit of Detection
Spectrum Analysis
Hot Temperature
Spectroscopy
Scattering
Molecules
Monitoring
Sensors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Kim, Uihan ; Song, Jaewoo ; Lee, Donghak ; Ryu, Suho ; Kim, Soocheol ; Hwang, Jaehyun ; Joo, Chulmin. / Capillary-scale direct measurement of hemoglobin concentration of erythrocytes using photothermal angular light scattering. In: Biosensors and Bioelectronics. 2015 ; Vol. 74. pp. 469-475.
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Capillary-scale direct measurement of hemoglobin concentration of erythrocytes using photothermal angular light scattering. / Kim, Uihan; Song, Jaewoo; Lee, Donghak; Ryu, Suho; Kim, Soocheol; Hwang, Jaehyun; Joo, Chulmin.

In: Biosensors and Bioelectronics, Vol. 74, 05.12.2015, p. 469-475.

Research output: Contribution to journalArticle

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