CaPUB1, a hot pepper U-box E3 ubiquitin ligase, confers enhanced cold stress tolerance and decreased drought stress tolerance in transgenic rice (Oryza sativa L.)

Hye Jo Min, Ye Jin Jung, Bin Goo Kang, Woo Taek Kim

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20 Citations (Scopus)

Abstract

Abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. Higher plants have developed various defense strategies against these unfavorable conditions. CaPUB1 (Capsicum annuum Putative U-box protein 1) is a hot pepper U-box E3 Ub ligase. Transgenic Arabidopsis plants that constitutively expressed CaPUB1 exhibited droughtsensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. In this study, CaPUB1 was over-expressed in rice (Oryza sativa L.), and the phenotypic properties of transgenic rice plants were examined in terms of their drought and cold stress tolerance. Ubi:CaPUB1 T3 transgenic rice plants displayed phenotypes hypersensitive to dehydration, suggesting that its role in the negative regulation of drought stress response is conserved in dicot Arabidopsis and monocot rice plants. In contrast, Ubi:CaPUB1 progeny exhibited phenotypes markedly tolerant to prolonged low temperature (4°C) treatment, compared to those of wild-type plants, as determined by survival rates, electrolyte leakage, and total chlorophyll content. Cold stress-induced marker genes, including DREB1A, DREB1B, DREB1C, and Cytochrome P450, were more up-regulated by cold treatment in Ubi:CaPUB1 plants than in wild-type plants. These results suggest that CaPUB1 serves as both a negative regulator of the drought stress response and a positive regulator of the cold stress response in transgenic rice plants. This raises the possibility that CaPUB1 participates in the cross-talk between drought and low-temperature signaling pathways.

Original languageEnglish
Pages (from-to)250-257
Number of pages8
JournalMolecules and cells
Volume39
Issue number3
DOIs
Publication statusPublished - 2016 Jan 1

Bibliographical note

Funding Information:
This work was supported by grants from the National Center for GM Crops (Project No.PJ01113801 funded by the Rural Development Administration, Republic of Korea) and the National Research Foundation (Project No. 2014R1A2A2A01003891) to W.T.K., and from the Korea Institute of Science and Technology Information to B.G.K.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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