Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene

Yangsoon Lee, Jinhwa Lee, Seokhoon Jeong, Jonghan Lee, Il Kwon Bae, Kyungwon Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objectives: To investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB. Methods: A total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes encoding OXA carbapenemases and metallo-β-lactamases was performed by PCR. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and repetitive-sequence-based PCR. The 450 bp sequence (zone 2) of the rpoB gene was amplified and sequenced. Results: Molecular characterization of the 272 CNSAB isolates identified five sequence types (STs): ST92, ST75, ST137, ST138 and ST69. The first four of these STs were clustered into clonal complex (CC) 92, sharing alleles at six of seven housekeeping gene loci; ST69 shared alleles at five of seven loci. CNSAB of CC92 carried the blaOXA-23 gene (n=169), the blaOXA-51-like gene preceded by ISAba1 (n=89) or both (n=14). Notably, all CNSAB isolates carried a G428T substitution in zone 2 of the rpoB gene. Conclusions: CNSAB isolates of CC92 with the G428T substitution in zone 2 of the rpoB gene are disseminated nationwide in Korea. A. baumannii with the single nucleotide substitution may be more likely to acquire carbapenem resistance than are other isolates.

Original languageEnglish
Article numberdkq402
Pages (from-to)66-72
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume66
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

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Acinetobacter baumannii
Carbapenems
Genes
Korea
Alleles
Multilocus Sequence Typing
Polymerase Chain Reaction
Acinetobacter
Nucleic Acid Repetitive Sequences
Essential Genes
Nucleotides

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

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title = "Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene",
abstract = "Objectives: To investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB. Methods: A total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes encoding OXA carbapenemases and metallo-β-lactamases was performed by PCR. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and repetitive-sequence-based PCR. The 450 bp sequence (zone 2) of the rpoB gene was amplified and sequenced. Results: Molecular characterization of the 272 CNSAB isolates identified five sequence types (STs): ST92, ST75, ST137, ST138 and ST69. The first four of these STs were clustered into clonal complex (CC) 92, sharing alleles at six of seven housekeeping gene loci; ST69 shared alleles at five of seven loci. CNSAB of CC92 carried the blaOXA-23 gene (n=169), the blaOXA-51-like gene preceded by ISAba1 (n=89) or both (n=14). Notably, all CNSAB isolates carried a G428T substitution in zone 2 of the rpoB gene. Conclusions: CNSAB isolates of CC92 with the G428T substitution in zone 2 of the rpoB gene are disseminated nationwide in Korea. A. baumannii with the single nucleotide substitution may be more likely to acquire carbapenem resistance than are other isolates.",
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Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene. / Lee, Yangsoon; Lee, Jinhwa; Jeong, Seokhoon; Lee, Jonghan; Bae, Il Kwon; Lee, Kyungwon.

In: Journal of Antimicrobial Chemotherapy, Vol. 66, No. 1, dkq402, 01.01.2011, p. 66-72.

Research output: Contribution to journalArticle

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AU - Lee, Yangsoon

AU - Lee, Jinhwa

AU - Jeong, Seokhoon

AU - Lee, Jonghan

AU - Bae, Il Kwon

AU - Lee, Kyungwon

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AB - Objectives: To investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB. Methods: A total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes encoding OXA carbapenemases and metallo-β-lactamases was performed by PCR. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and repetitive-sequence-based PCR. The 450 bp sequence (zone 2) of the rpoB gene was amplified and sequenced. Results: Molecular characterization of the 272 CNSAB isolates identified five sequence types (STs): ST92, ST75, ST137, ST138 and ST69. The first four of these STs were clustered into clonal complex (CC) 92, sharing alleles at six of seven housekeeping gene loci; ST69 shared alleles at five of seven loci. CNSAB of CC92 carried the blaOXA-23 gene (n=169), the blaOXA-51-like gene preceded by ISAba1 (n=89) or both (n=14). Notably, all CNSAB isolates carried a G428T substitution in zone 2 of the rpoB gene. Conclusions: CNSAB isolates of CC92 with the G428T substitution in zone 2 of the rpoB gene are disseminated nationwide in Korea. A. baumannii with the single nucleotide substitution may be more likely to acquire carbapenem resistance than are other isolates.

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