Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesis

Sungwoon Lee, Seung Sik Rho, Hyojin Park, Jeong Ae Park, Jihye Kim, In Kyu Lee, Gou Young Koh, Naoki Mochizuki, Young Myeong Kim, Young-Guen Kwon

Research output: Contribution to journalArticle

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Abstract

Controlled angiogenesis and lymphangiogenesis are essential for tissue development, function, and repair. However, aberrant neovascularization is an essential pathogenic mechanism in many human diseases, including diseases involving tumor growth and survival. Here, we have demonstrated that mice deficient in C-type lectin family 14 member A (CLEC14A) display enhanced angiogenic sprouting and hemorrhage as well as enlarged jugular lymph sacs and lymphatic vessels. CLEC14A formed a complex with VEGFR-3 in endothelial cells (ECs), and CLEC14A KO resulted in a marked reduction in VEGFR-3 that was concomitant with increases in VEGFR-2 expression and downstream signaling. Implanted tumor growth was profoundly reduced in CLEC14A-KO mice compared with that seen in WT littermates, but tumor-bearing CLEC14A-KO mice died sooner. Tumors in CLEC14A-KO mice had increased numbers of nonfunctional blood vessels and severe hemorrhaging. Blockade of VEGFR-2 signaling suppressed these vascular abnormalities and enhanced the survival of tumor-bearing CLEC14A-KO mice. We conclude that CLEC14A acts in vascular homeostasis by fine-tuning VEGFR-2 and VEGFR-3 signaling in ECs, suggesting its relevance in the pathogenesis of angiogenesis-related human disorders.

Original languageEnglish
Pages (from-to)457-471
Number of pages15
JournalJournal of Clinical Investigation
Volume127
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

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Vascular Endothelial Growth Factor Receptor-3
Lymphangiogenesis
C-Type Lectins
Vascular Endothelial Growth Factor Receptor-2
Protein C
Blood Vessels
Neoplasms
Endothelial Cells
Lymphatic Vessels
Survival
saccharide-binding proteins
Lymph
Growth
Homeostasis
Neck
Hemorrhage

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, Sungwoon ; Rho, Seung Sik ; Park, Hyojin ; Park, Jeong Ae ; Kim, Jihye ; Lee, In Kyu ; Koh, Gou Young ; Mochizuki, Naoki ; Kim, Young Myeong ; Kwon, Young-Guen. / Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesis. In: Journal of Clinical Investigation. 2017 ; Vol. 127, No. 2. pp. 457-471.
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Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesis. / Lee, Sungwoon; Rho, Seung Sik; Park, Hyojin; Park, Jeong Ae; Kim, Jihye; Lee, In Kyu; Koh, Gou Young; Mochizuki, Naoki; Kim, Young Myeong; Kwon, Young-Guen.

In: Journal of Clinical Investigation, Vol. 127, No. 2, 01.02.2017, p. 457-471.

Research output: Contribution to journalArticle

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AU - Lee, Sungwoon

AU - Rho, Seung Sik

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AU - Park, Jeong Ae

AU - Kim, Jihye

AU - Lee, In Kyu

AU - Koh, Gou Young

AU - Mochizuki, Naoki

AU - Kim, Young Myeong

AU - Kwon, Young-Guen

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N2 - Controlled angiogenesis and lymphangiogenesis are essential for tissue development, function, and repair. However, aberrant neovascularization is an essential pathogenic mechanism in many human diseases, including diseases involving tumor growth and survival. Here, we have demonstrated that mice deficient in C-type lectin family 14 member A (CLEC14A) display enhanced angiogenic sprouting and hemorrhage as well as enlarged jugular lymph sacs and lymphatic vessels. CLEC14A formed a complex with VEGFR-3 in endothelial cells (ECs), and CLEC14A KO resulted in a marked reduction in VEGFR-3 that was concomitant with increases in VEGFR-2 expression and downstream signaling. Implanted tumor growth was profoundly reduced in CLEC14A-KO mice compared with that seen in WT littermates, but tumor-bearing CLEC14A-KO mice died sooner. Tumors in CLEC14A-KO mice had increased numbers of nonfunctional blood vessels and severe hemorrhaging. Blockade of VEGFR-2 signaling suppressed these vascular abnormalities and enhanced the survival of tumor-bearing CLEC14A-KO mice. We conclude that CLEC14A acts in vascular homeostasis by fine-tuning VEGFR-2 and VEGFR-3 signaling in ECs, suggesting its relevance in the pathogenesis of angiogenesis-related human disorders.

AB - Controlled angiogenesis and lymphangiogenesis are essential for tissue development, function, and repair. However, aberrant neovascularization is an essential pathogenic mechanism in many human diseases, including diseases involving tumor growth and survival. Here, we have demonstrated that mice deficient in C-type lectin family 14 member A (CLEC14A) display enhanced angiogenic sprouting and hemorrhage as well as enlarged jugular lymph sacs and lymphatic vessels. CLEC14A formed a complex with VEGFR-3 in endothelial cells (ECs), and CLEC14A KO resulted in a marked reduction in VEGFR-3 that was concomitant with increases in VEGFR-2 expression and downstream signaling. Implanted tumor growth was profoundly reduced in CLEC14A-KO mice compared with that seen in WT littermates, but tumor-bearing CLEC14A-KO mice died sooner. Tumors in CLEC14A-KO mice had increased numbers of nonfunctional blood vessels and severe hemorrhaging. Blockade of VEGFR-2 signaling suppressed these vascular abnormalities and enhanced the survival of tumor-bearing CLEC14A-KO mice. We conclude that CLEC14A acts in vascular homeostasis by fine-tuning VEGFR-2 and VEGFR-3 signaling in ECs, suggesting its relevance in the pathogenesis of angiogenesis-related human disorders.

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