Abstract
In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technology has been widely employed for rapid analysis of the glycan binding properties of lectins and antibodies, the quantitative measurements of glycan-protein interactions, detection of cells and pathogens, identification of disease-related anti-glycan antibodies for diagnosis, and fast assessment of substrate specificities of glycosyltransferases. This review covers the construction of carbohydrate microarrays, detection methods of carbohydrate microarrays and their applications in biological and biomedical research.
| Original language | English |
|---|---|
| Pages (from-to) | 4310-4326 |
| Number of pages | 17 |
| Journal | Chemical Society Reviews |
| Volume | 42 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2013 May 21 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Carbohydrate microarrays. / Park, Sungjin; Gildersleeve, Jeffrey C.; Blixt, Ola; Shin, Injae.
In: Chemical Society Reviews, Vol. 42, No. 10, 21.05.2013, p. 4310-4326.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Carbohydrate microarrays
AU - Park, Sungjin
AU - Gildersleeve, Jeffrey C.
AU - Blixt, Ola
AU - Shin, Injae
PY - 2013/5/21
Y1 - 2013/5/21
N2 - In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technology has been widely employed for rapid analysis of the glycan binding properties of lectins and antibodies, the quantitative measurements of glycan-protein interactions, detection of cells and pathogens, identification of disease-related anti-glycan antibodies for diagnosis, and fast assessment of substrate specificities of glycosyltransferases. This review covers the construction of carbohydrate microarrays, detection methods of carbohydrate microarrays and their applications in biological and biomedical research.
AB - In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technology has been widely employed for rapid analysis of the glycan binding properties of lectins and antibodies, the quantitative measurements of glycan-protein interactions, detection of cells and pathogens, identification of disease-related anti-glycan antibodies for diagnosis, and fast assessment of substrate specificities of glycosyltransferases. This review covers the construction of carbohydrate microarrays, detection methods of carbohydrate microarrays and their applications in biological and biomedical research.
UR - http://www.scopus.com/inward/record.url?scp=84876913552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876913552&partnerID=8YFLogxK
U2 - 10.1039/c2cs35401b
DO - 10.1039/c2cs35401b
M3 - Review article
VL - 42
SP - 4310
EP - 4326
JO - Chemical Society Reviews
JF - Chemical Society Reviews
SN - 0306-0012
IS - 10
ER -