Carbohydrate microarrays containing glycosylated fluorescent probes for assessment of glycosidase activities

Ji Young Hyun, Na Rae Kang, Injae Shin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Carbohydrate microarrays, containing glycosylated fluorescent probes, have been constructed using N-hydroxysuccinimide (NHS) ester-conjugated BSA modified surfaces. When the carbohydrate moieties were cleaved from the fluorescent probes in the conjugates by glycosidases, the fluorescence signals of the probes were enhanced. In this study, we have applied these microarrays to profile glycosidase activities and have employed them to determine IC50 values of glycosidase inhibitors.

Original languageEnglish
Pages (from-to)1240-1243
Number of pages4
JournalOrganic Letters
Volume20
Issue number4
DOIs
Publication statusPublished - 2018 Feb 16

Fingerprint

Glycoside Hydrolases
carbohydrates
Microarrays
Fluorescent Dyes
Carbohydrates
probes
inhibitors
Inhibitory Concentration 50
esters
Esters
Fluorescence
fluorescence
profiles

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

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Carbohydrate microarrays containing glycosylated fluorescent probes for assessment of glycosidase activities. / Hyun, Ji Young; Kang, Na Rae; Shin, Injae.

In: Organic Letters, Vol. 20, No. 4, 16.02.2018, p. 1240-1243.

Research output: Contribution to journalArticle

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AU - Shin, Injae

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