Carbohydrate microarrays for screening functional glycans

Jaeyoung Pai, Ji Young Hyun, Jieun Jeong, Sohee Loh, Eun Hee Cho, Young Sun Kang, Injae Shin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Carbohydrate microarrays have become robust and powerful tools for the rapid analysis of glycan-associated binding events. However, this microarray technology has rarely been applied in studies of glycan-mediated cellular responses. Herein we describe a carbohydrate microarray-based approach for the rapid screening of biologically active glycans that stimulate the production of reactive oxygen species (ROS) through binding to the cell-surface lectin. We employed a microarray assay and a fluorescent ROS probe to identify the functional glycans which enhance ROS production. Cells binding to glycans on the microarrays produced ROS, whose levels were decreased in the presence of a ROS scavenger or a NADPH oxidase inhibitor. The present study leads us to suggest that glycan microarrays are applicable to the simultaneous screening of various glycans whose binding to the cell-surface lectin elicits cellular response.

Original languageEnglish
Pages (from-to)2084-2093
Number of pages10
JournalChemical Science
Volume7
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Carbohydrate microarrays for screening functional glycans'. Together they form a unique fingerprint.

  • Cite this

    Pai, J., Hyun, J. Y., Jeong, J., Loh, S., Cho, E. H., Kang, Y. S., & Shin, I. (2016). Carbohydrate microarrays for screening functional glycans. Chemical Science, 7(3), 2084-2093. https://doi.org/10.1039/c5sc03789a