Carbohydrate microarrays for screening functional glycans

Jaeyoung Pai, Ji Young Hyun, Jieun Jeong, Sohee Loh, Eun Hee Cho, Young Sun Kang, Injae Shin

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Carbohydrate microarrays have become robust and powerful tools for the rapid analysis of glycan-associated binding events. However, this microarray technology has rarely been applied in studies of glycan-mediated cellular responses. Herein we describe a carbohydrate microarray-based approach for the rapid screening of biologically active glycans that stimulate the production of reactive oxygen species (ROS) through binding to the cell-surface lectin. We employed a microarray assay and a fluorescent ROS probe to identify the functional glycans which enhance ROS production. Cells binding to glycans on the microarrays produced ROS, whose levels were decreased in the presence of a ROS scavenger or a NADPH oxidase inhibitor. The present study leads us to suggest that glycan microarrays are applicable to the simultaneous screening of various glycans whose binding to the cell-surface lectin elicits cellular response.

Original languageEnglish
Pages (from-to)2084-2093
Number of pages10
JournalChemical Science
Volume7
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry 2016.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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