Carbohydrate microarrays for screening functional glycans

Jaeyoung Pai, Ji Young Hyun, Jieun Jeong, Sohee Loh, Eun Hee Cho, Young Sun Kang, Injae Shin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Carbohydrate microarrays have become robust and powerful tools for the rapid analysis of glycan-associated binding events. However, this microarray technology has rarely been applied in studies of glycan-mediated cellular responses. Herein we describe a carbohydrate microarray-based approach for the rapid screening of biologically active glycans that stimulate the production of reactive oxygen species (ROS) through binding to the cell-surface lectin. We employed a microarray assay and a fluorescent ROS probe to identify the functional glycans which enhance ROS production. Cells binding to glycans on the microarrays produced ROS, whose levels were decreased in the presence of a ROS scavenger or a NADPH oxidase inhibitor. The present study leads us to suggest that glycan microarrays are applicable to the simultaneous screening of various glycans whose binding to the cell-surface lectin elicits cellular response.

Original languageEnglish
Pages (from-to)2084-2093
Number of pages10
JournalChemical Science
Volume7
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

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Microarrays
Polysaccharides
Screening
Carbohydrates
Reactive Oxygen Species
Lectins
NADPH Oxidase
Assays

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Pai, J., Hyun, J. Y., Jeong, J., Loh, S., Cho, E. H., Kang, Y. S., & Shin, I. (2016). Carbohydrate microarrays for screening functional glycans. Chemical Science, 7(3), 2084-2093. https://doi.org/10.1039/c5sc03789a
Pai, Jaeyoung ; Hyun, Ji Young ; Jeong, Jieun ; Loh, Sohee ; Cho, Eun Hee ; Kang, Young Sun ; Shin, Injae. / Carbohydrate microarrays for screening functional glycans. In: Chemical Science. 2016 ; Vol. 7, No. 3. pp. 2084-2093.
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Pai, J, Hyun, JY, Jeong, J, Loh, S, Cho, EH, Kang, YS & Shin, I 2016, 'Carbohydrate microarrays for screening functional glycans', Chemical Science, vol. 7, no. 3, pp. 2084-2093. https://doi.org/10.1039/c5sc03789a

Carbohydrate microarrays for screening functional glycans. / Pai, Jaeyoung; Hyun, Ji Young; Jeong, Jieun; Loh, Sohee; Cho, Eun Hee; Kang, Young Sun; Shin, Injae.

In: Chemical Science, Vol. 7, No. 3, 01.03.2016, p. 2084-2093.

Research output: Contribution to journalArticle

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Pai J, Hyun JY, Jeong J, Loh S, Cho EH, Kang YS et al. Carbohydrate microarrays for screening functional glycans. Chemical Science. 2016 Mar 1;7(3):2084-2093. https://doi.org/10.1039/c5sc03789a