Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

Sung Ung Moon, Jihee Kim, Kiran Kumar Bokara, Jong Youl Kim, Dongwoo Khang, Thomas J. Webster, Jongeun Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c [OX-42]). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention. 2012 Cárdenas et al, publisher and licensee Dove Medical Press Ltd.

Original languageEnglish
Pages (from-to)2751-2765
Number of pages15
JournalInternational journal of nanomedicine
Volume7
DOIs
Publication statusPublished - 2012 Dec 5

Fingerprint

Carbon Nanotubes
Lateral Ventricles
Carbon nanotubes
Stem Cells
Stroke
Recovery
Transplants
Neurons
Rats
Proteins
Cysts
Nestin
Synaptophysin
Microtubule-Associated Proteins
Transplantation
Glial Fibrillary Acidic Protein
Bromodeoxyuridine
Stem cells
Repair
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Moon, Sung Ung ; Kim, Jihee ; Bokara, Kiran Kumar ; Kim, Jong Youl ; Khang, Dongwoo ; Webster, Thomas J. ; Lee, Jongeun. / Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke. In: International journal of nanomedicine. 2012 ; Vol. 7. pp. 2751-2765.
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abstract = "The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c [OX-42]). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention. 2012 C{\'a}rdenas et al, publisher and licensee Dove Medical Press Ltd.",
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Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke. / Moon, Sung Ung; Kim, Jihee; Bokara, Kiran Kumar; Kim, Jong Youl; Khang, Dongwoo; Webster, Thomas J.; Lee, Jongeun.

In: International journal of nanomedicine, Vol. 7, 05.12.2012, p. 2751-2765.

Research output: Contribution to journalArticle

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