Carbonic anhydrase-mimetic bolaamphiphile self-assembly for CO2 hydration and sequestration

Min Chul Kim, Sang Yup Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A biomimetic catalyst was prepared through the self-assembly of a bolaamphiphilic molecule with histidine moieties for the sequestration of carbon dioxide. The histidyl bolaamphiphilic molecule bis(N-a-amidohistidine)-1,7-heptane dicarboxylate has been synthesized and self-assembled to produce analogues of the active sites of carbonic anhydrase (CA) after association with Zn2+ ions. Spectroscopic analysis demonstrated the coordination of the Zn2+ ions with histidine imidazole moieties, which is the core conformation of CA active sites. The Zn-associated self-assembly worked as a CA-mimetic catalyst that shows catalytic activity for CO2 hydration. Evaluation of the kinetics of using para-nitrophenylacetate revealed that the kinetic parameters of the CA-mimetic catalyst were maximized at the optimal Zn concentration and that excess Zn ions resulted in deteriorated catalytic activity. The performance of the CA-mimetic catalyst was enhanced by changing the pH value and temperature of the reaction, which implies that the hydrolysis of the substrate is the rate-determining step. The catalyst-assisted sequestration of CO2 was demonstrated by CaCO3 precipitation upon the addition of Ca2+ ions. This study offers an easy way to prepare enzyme analogues for CO2 sequestration through the self-assembly of bolaamphiphile molecules with designer biochemical moieties.

Original languageEnglish
Pages (from-to)17019-17024
Number of pages6
JournalChemistry - A European Journal
Volume20
Issue number51
DOIs
Publication statusPublished - 2014 Dec 15

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Carbonic anhydrase
Carbonic Anhydrases
Hydration
Self assembly
Catalysts
Ions
Histidine
Molecules
Catalyst activity
Heptanes
Spectroscopic analysis
Biomimetics
Heptane
Kinetic parameters
Carbon Dioxide
Conformations
Hydrolysis
Carbon dioxide
Enzymes
bolaamphiphile

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Organic Chemistry

Cite this

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abstract = "A biomimetic catalyst was prepared through the self-assembly of a bolaamphiphilic molecule with histidine moieties for the sequestration of carbon dioxide. The histidyl bolaamphiphilic molecule bis(N-a-amidohistidine)-1,7-heptane dicarboxylate has been synthesized and self-assembled to produce analogues of the active sites of carbonic anhydrase (CA) after association with Zn2+ ions. Spectroscopic analysis demonstrated the coordination of the Zn2+ ions with histidine imidazole moieties, which is the core conformation of CA active sites. The Zn-associated self-assembly worked as a CA-mimetic catalyst that shows catalytic activity for CO2 hydration. Evaluation of the kinetics of using para-nitrophenylacetate revealed that the kinetic parameters of the CA-mimetic catalyst were maximized at the optimal Zn concentration and that excess Zn ions resulted in deteriorated catalytic activity. The performance of the CA-mimetic catalyst was enhanced by changing the pH value and temperature of the reaction, which implies that the hydrolysis of the substrate is the rate-determining step. The catalyst-assisted sequestration of CO2 was demonstrated by CaCO3 precipitation upon the addition of Ca2+ ions. This study offers an easy way to prepare enzyme analogues for CO2 sequestration through the self-assembly of bolaamphiphile molecules with designer biochemical moieties.",
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Carbonic anhydrase-mimetic bolaamphiphile self-assembly for CO2 hydration and sequestration. / Kim, Min Chul; Lee, Sang Yup.

In: Chemistry - A European Journal, Vol. 20, No. 51, 15.12.2014, p. 17019-17024.

Research output: Contribution to journalArticle

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