Abstract
Triglyceride (TG) accumulation causes macrophage cell death, which affects the development of atherosclerosis. Here, we examined whether caspase-2 is implicated in TG-induced macrophage cell death. We found that caspase-2 activity is increased in TG-treated THP-1 macrophages, and that inhibition of caspase-2 activity drastically inhibits TG-induced cell death. We previously reported that TG-induced macrophage cell death is triggered by caspase-1, and thus investigated the relationship between caspase-2 and caspase-1 in TG-induced macrophage cell death. Inhibition of caspase-2 activity decreased caspase-1 activity in TG-treated macrophages. However, caspase-1 inhibition did not affect caspase-2 activity, suggesting that caspase-2 is upstream of caspase-1. Furthermore, we found that TG induces activation of caspase-3, -7, -8, and -9, as well as cleavage of PARP. Inhibition of caspase-2 and -1 decreased TG-induced caspase-3, -7, -8, and -9 activation and PARP cleavage. Taken together, these results suggest that TG-induced macrophage cell death is mediated via the caspase-2/caspase-1/apoptotic caspases/PARP pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 510-515 |
| Number of pages | 6 |
| Journal | BMB reports |
| Volume | 50 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
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Caspase-2 mediates triglyceride (TG)-induced macrophage cell death. / Lim, Jaewon; Kim, Hyun Kyung; Kim, Sung Hoon; Rhee, Ki Jong; Kim, Yoon Suk.
In: BMB reports, Vol. 50, No. 10, 01.01.2017, p. 510-515.Research output: Contribution to journal › Article
TY - JOUR
T1 - Caspase-2 mediates triglyceride (TG)-induced macrophage cell death
AU - Lim, Jaewon
AU - Kim, Hyun Kyung
AU - Kim, Sung Hoon
AU - Rhee, Ki Jong
AU - Kim, Yoon Suk
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Triglyceride (TG) accumulation causes macrophage cell death, which affects the development of atherosclerosis. Here, we examined whether caspase-2 is implicated in TG-induced macrophage cell death. We found that caspase-2 activity is increased in TG-treated THP-1 macrophages, and that inhibition of caspase-2 activity drastically inhibits TG-induced cell death. We previously reported that TG-induced macrophage cell death is triggered by caspase-1, and thus investigated the relationship between caspase-2 and caspase-1 in TG-induced macrophage cell death. Inhibition of caspase-2 activity decreased caspase-1 activity in TG-treated macrophages. However, caspase-1 inhibition did not affect caspase-2 activity, suggesting that caspase-2 is upstream of caspase-1. Furthermore, we found that TG induces activation of caspase-3, -7, -8, and -9, as well as cleavage of PARP. Inhibition of caspase-2 and -1 decreased TG-induced caspase-3, -7, -8, and -9 activation and PARP cleavage. Taken together, these results suggest that TG-induced macrophage cell death is mediated via the caspase-2/caspase-1/apoptotic caspases/PARP pathways.
AB - Triglyceride (TG) accumulation causes macrophage cell death, which affects the development of atherosclerosis. Here, we examined whether caspase-2 is implicated in TG-induced macrophage cell death. We found that caspase-2 activity is increased in TG-treated THP-1 macrophages, and that inhibition of caspase-2 activity drastically inhibits TG-induced cell death. We previously reported that TG-induced macrophage cell death is triggered by caspase-1, and thus investigated the relationship between caspase-2 and caspase-1 in TG-induced macrophage cell death. Inhibition of caspase-2 activity decreased caspase-1 activity in TG-treated macrophages. However, caspase-1 inhibition did not affect caspase-2 activity, suggesting that caspase-2 is upstream of caspase-1. Furthermore, we found that TG induces activation of caspase-3, -7, -8, and -9, as well as cleavage of PARP. Inhibition of caspase-2 and -1 decreased TG-induced caspase-3, -7, -8, and -9 activation and PARP cleavage. Taken together, these results suggest that TG-induced macrophage cell death is mediated via the caspase-2/caspase-1/apoptotic caspases/PARP pathways.
UR - http://www.scopus.com/inward/record.url?scp=85032466143&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032466143&partnerID=8YFLogxK
U2 - 10.5483/BMBRep.2017.50.10.106
DO - 10.5483/BMBRep.2017.50.10.106
M3 - Article
C2 - 28768565
AN - SCOPUS:85032466143
VL - 50
SP - 510
EP - 515
JO - BMB Reports
JF - BMB Reports
SN - 1976-6696
IS - 10
ER -