Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells

Zang Hee Lee, Shee Eun Lee, Kyubum Kwack, Whanho Yeo, Tae Ho Lee, Sun Sik Bae, Pann Ghill Suh, Hong Hee Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The tumor necrosis factor receptor (TNFR)-associated factor (TRAF) proteins play a central role in the early steps of signal transduction by TNFR superfamily proteins, which induce various cellular responses, including apoptosis. Influences of TRAF proteins on the regulation of cell death and physical interactions between TRAFs and caspases have been reported. In this study, we demonstrate that TRAF3 is proteolyzed during cell death in a caspase-dependent manner. TRAF3 was found to be cleaved by incubation with caspase3 in vitro and by Fas- or CD3-triggering in Jurkat-T cells. The Fas- or CD3-induced cleavage of TRAF3 was blocked by caspase inhibitors and by introduction of alanine substitutions for D347 and D367 residues. Furthermore, the amino-terminal fragment of TRAF3 showed a different intracellular localization from the full-length TRAF3 with preferential distribution to particulate fractions and the nucleus. These findings suggest that TRAF3 may be regulated by caspases during apoptosis of T cells.

Original languageEnglish
Pages (from-to)490-496
Number of pages7
JournalJournal of Leukocyte Biology
Volume69
Issue number3
Publication statusPublished - 2001 Mar 28

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TNF Receptor-Associated Factor 3
Jurkat Cells
Caspases
T-Lymphocytes
Cell Death
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Apoptosis
Proteins
Caspase Inhibitors
Tumor Necrosis Factor Receptors
Alanine
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Lee, Z. H., Lee, S. E., Kwack, K., Yeo, W., Lee, T. H., Bae, S. S., ... Kim, H. H. (2001). Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells. Journal of Leukocyte Biology, 69(3), 490-496.
Lee, Zang Hee ; Lee, Shee Eun ; Kwack, Kyubum ; Yeo, Whanho ; Lee, Tae Ho ; Bae, Sun Sik ; Suh, Pann Ghill ; Kim, Hong Hee. / Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells. In: Journal of Leukocyte Biology. 2001 ; Vol. 69, No. 3. pp. 490-496.
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Lee, ZH, Lee, SE, Kwack, K, Yeo, W, Lee, TH, Bae, SS, Suh, PG & Kim, HH 2001, 'Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells', Journal of Leukocyte Biology, vol. 69, no. 3, pp. 490-496.

Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells. / Lee, Zang Hee; Lee, Shee Eun; Kwack, Kyubum; Yeo, Whanho; Lee, Tae Ho; Bae, Sun Sik; Suh, Pann Ghill; Kim, Hong Hee.

In: Journal of Leukocyte Biology, Vol. 69, No. 3, 28.03.2001, p. 490-496.

Research output: Contribution to journalArticle

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Lee ZH, Lee SE, Kwack K, Yeo W, Lee TH, Bae SS et al. Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells. Journal of Leukocyte Biology. 2001 Mar 28;69(3):490-496.