Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers

huinam pak, Yong Seog Oh, Yen Bin Liu, Tsu Juey Wu, Hrayr S. Karagueuzian, Shien Fong Lin, Peng Sheng Chen

Research output: Contribution to journalArticle

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Abstract

Background-A therapeutic implication of the focal-source hypothesis of ventricular fibrillation (VF) is that VF can be terminated by focal ablation. We hypothesize that β-adrenergic receptor blockade converts multiple-wavelet VF to focal-source VF and that this focal source is located near the papillary muscle (PM). Methods and Results-We used optical mapping techniques to study the effects of propranolol (0.3 mg/L) on VF dynamics in Langendorff-perfused rabbit hearts. The left ventricular (LV) anterior wall was mapped and optical action potential duration restitution (APDR) was determined at 25 epicardial sites. We performed ablation during VF of the left anterior PM in hearts with (N=6) or without (N=6) cytochalasin infusion, the LV lateral epicardium (Epi group, N=3), and the LV endocardium (Endo group, N=3). The PM was also ablated in 3 hearts without propranolol (control group). Propranolol converted multiple-wavelet VF to slow VF with reentry localized to the PM. Propranolol decreased the maximal slope of the APDR curve (P<0.001) as well as its spatial heterogeneity (P<0.01) and conduction velocity (P=0.01) while increasing the VF cycle length (P<0.001). PM ablation terminated VF during propropranolol infusion with (6 of 6, 100%) or without (4 of 6, 67%) cytochalasin D and significantly reduced inducibility. VF did not terminate in the Epi, Endo, and control groups (P<0.001). Conclusions-Propranolol flattens the APDR curve and reduces conduction velocity, converting multiple-wavelet VF into VF with a focal source anchored to the PM. Ablation of this focal source may terminate VF.

Original languageEnglish
Pages (from-to)3149-3156
Number of pages8
JournalCirculation
Volume108
Issue number25
DOIs
Publication statusPublished - 2003 Dec 23

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Catheter Ablation
Ventricular Fibrillation
Rabbits
Papillary Muscles
Propranolol
Action Potentials
Cytochalasins
Cytochalasin D
Endocardium
Control Groups
Pericardium
Adrenergic Receptors

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

pak, H., Oh, Y. S., Liu, Y. B., Wu, T. J., Karagueuzian, H. S., Lin, S. F., & Chen, P. S. (2003). Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers. Circulation, 108(25), 3149-3156. https://doi.org/10.1161/01.CIR.0000104563.12408.12
pak, huinam ; Oh, Yong Seog ; Liu, Yen Bin ; Wu, Tsu Juey ; Karagueuzian, Hrayr S. ; Lin, Shien Fong ; Chen, Peng Sheng. / Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers. In: Circulation. 2003 ; Vol. 108, No. 25. pp. 3149-3156.
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abstract = "Background-A therapeutic implication of the focal-source hypothesis of ventricular fibrillation (VF) is that VF can be terminated by focal ablation. We hypothesize that β-adrenergic receptor blockade converts multiple-wavelet VF to focal-source VF and that this focal source is located near the papillary muscle (PM). Methods and Results-We used optical mapping techniques to study the effects of propranolol (0.3 mg/L) on VF dynamics in Langendorff-perfused rabbit hearts. The left ventricular (LV) anterior wall was mapped and optical action potential duration restitution (APDR) was determined at 25 epicardial sites. We performed ablation during VF of the left anterior PM in hearts with (N=6) or without (N=6) cytochalasin infusion, the LV lateral epicardium (Epi group, N=3), and the LV endocardium (Endo group, N=3). The PM was also ablated in 3 hearts without propranolol (control group). Propranolol converted multiple-wavelet VF to slow VF with reentry localized to the PM. Propranolol decreased the maximal slope of the APDR curve (P<0.001) as well as its spatial heterogeneity (P<0.01) and conduction velocity (P=0.01) while increasing the VF cycle length (P<0.001). PM ablation terminated VF during propropranolol infusion with (6 of 6, 100{\%}) or without (4 of 6, 67{\%}) cytochalasin D and significantly reduced inducibility. VF did not terminate in the Epi, Endo, and control groups (P<0.001). Conclusions-Propranolol flattens the APDR curve and reduces conduction velocity, converting multiple-wavelet VF into VF with a focal source anchored to the PM. Ablation of this focal source may terminate VF.",
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pak, H, Oh, YS, Liu, YB, Wu, TJ, Karagueuzian, HS, Lin, SF & Chen, PS 2003, 'Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers', Circulation, vol. 108, no. 25, pp. 3149-3156. https://doi.org/10.1161/01.CIR.0000104563.12408.12

Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers. / pak, huinam; Oh, Yong Seog; Liu, Yen Bin; Wu, Tsu Juey; Karagueuzian, Hrayr S.; Lin, Shien Fong; Chen, Peng Sheng.

In: Circulation, Vol. 108, No. 25, 23.12.2003, p. 3149-3156.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Catheter Ablation of Ventricular Fibrillation in Rabbit Ventricles Treated with β-Blockers

AU - pak, huinam

AU - Oh, Yong Seog

AU - Liu, Yen Bin

AU - Wu, Tsu Juey

AU - Karagueuzian, Hrayr S.

AU - Lin, Shien Fong

AU - Chen, Peng Sheng

PY - 2003/12/23

Y1 - 2003/12/23

N2 - Background-A therapeutic implication of the focal-source hypothesis of ventricular fibrillation (VF) is that VF can be terminated by focal ablation. We hypothesize that β-adrenergic receptor blockade converts multiple-wavelet VF to focal-source VF and that this focal source is located near the papillary muscle (PM). Methods and Results-We used optical mapping techniques to study the effects of propranolol (0.3 mg/L) on VF dynamics in Langendorff-perfused rabbit hearts. The left ventricular (LV) anterior wall was mapped and optical action potential duration restitution (APDR) was determined at 25 epicardial sites. We performed ablation during VF of the left anterior PM in hearts with (N=6) or without (N=6) cytochalasin infusion, the LV lateral epicardium (Epi group, N=3), and the LV endocardium (Endo group, N=3). The PM was also ablated in 3 hearts without propranolol (control group). Propranolol converted multiple-wavelet VF to slow VF with reentry localized to the PM. Propranolol decreased the maximal slope of the APDR curve (P<0.001) as well as its spatial heterogeneity (P<0.01) and conduction velocity (P=0.01) while increasing the VF cycle length (P<0.001). PM ablation terminated VF during propropranolol infusion with (6 of 6, 100%) or without (4 of 6, 67%) cytochalasin D and significantly reduced inducibility. VF did not terminate in the Epi, Endo, and control groups (P<0.001). Conclusions-Propranolol flattens the APDR curve and reduces conduction velocity, converting multiple-wavelet VF into VF with a focal source anchored to the PM. Ablation of this focal source may terminate VF.

AB - Background-A therapeutic implication of the focal-source hypothesis of ventricular fibrillation (VF) is that VF can be terminated by focal ablation. We hypothesize that β-adrenergic receptor blockade converts multiple-wavelet VF to focal-source VF and that this focal source is located near the papillary muscle (PM). Methods and Results-We used optical mapping techniques to study the effects of propranolol (0.3 mg/L) on VF dynamics in Langendorff-perfused rabbit hearts. The left ventricular (LV) anterior wall was mapped and optical action potential duration restitution (APDR) was determined at 25 epicardial sites. We performed ablation during VF of the left anterior PM in hearts with (N=6) or without (N=6) cytochalasin infusion, the LV lateral epicardium (Epi group, N=3), and the LV endocardium (Endo group, N=3). The PM was also ablated in 3 hearts without propranolol (control group). Propranolol converted multiple-wavelet VF to slow VF with reentry localized to the PM. Propranolol decreased the maximal slope of the APDR curve (P<0.001) as well as its spatial heterogeneity (P<0.01) and conduction velocity (P=0.01) while increasing the VF cycle length (P<0.001). PM ablation terminated VF during propropranolol infusion with (6 of 6, 100%) or without (4 of 6, 67%) cytochalasin D and significantly reduced inducibility. VF did not terminate in the Epi, Endo, and control groups (P<0.001). Conclusions-Propranolol flattens the APDR curve and reduces conduction velocity, converting multiple-wavelet VF into VF with a focal source anchored to the PM. Ablation of this focal source may terminate VF.

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