Surface-modified cellulose nanocrystals (CNCs) were developed for efficient delivery of polymeric siRNA in cancer cells. Cationic CNCs were synthesized using the sequential process of hydrothermal desulfation and chemical modification following which, polymeric siRNA obtained using from a two-step process of rolling circle transcription and Mg2+ chelation was complexed with the modified CNCs by electrostatic interaction. The complexation efficiency was optimized for high drug loading and release in the cytoplasmic environment. The resultant nanocomplex showed significantly enhanced enzymatic stability, gene knockdown efficacy, and apoptosis-induced in vitro therapeutic effect. Our results suggest CNCs as a promising carbohydrate-based delivery platform which could be utilized for RNAi-mediated cancer therapeutics.
Bibliographical noteFunding Information:
This study was supported by the Basic Science Research Program (No. 2018R1D1A1A02085552 and 2017R1A6A3A11029462 ) through the National Research Foundation of Korea (NRF) funded by the Korean Government . The work was supported in part by Brain Korea 21 ( BK21 ) PLUS program. This research was also partially supported by the Graduate School of YONSEI University Research Scholarship Grants in 2019 .
© 2020 Elsevier Ltd
All Science Journal Classification (ASJC) codes
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry