Abstract
It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30-2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1-100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making ∼ 3 nm holes in living cell membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 11179-11185 |
| Number of pages | 7 |
| Journal | Journal of Physical Chemistry B |
| Volume | 113 |
| Issue number | 32 |
| DOIs | |
| Publication status | Published - 2009 Aug 13 |
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All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Materials Chemistry
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Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes. / Chen, Jiumei; Hessler, Jessica A.; Putchakayala, Krishna; Panama, Brian K.; Khan, Damian P.; Hong, Seungpyo; Mullen, Douglas G.; DiMaggio, Stassi C.; Som, Abhigyan; Tew, Gregory N.; Lopatin, Anatoli N.; Baker, James R.; Holl, Mark M.Banaszak; Orr, Bradford G.
In: Journal of Physical Chemistry B, Vol. 113, No. 32, 13.08.2009, p. 11179-11185.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes
AU - Chen, Jiumei
AU - Hessler, Jessica A.
AU - Putchakayala, Krishna
AU - Panama, Brian K.
AU - Khan, Damian P.
AU - Hong, Seungpyo
AU - Mullen, Douglas G.
AU - DiMaggio, Stassi C.
AU - Som, Abhigyan
AU - Tew, Gregory N.
AU - Lopatin, Anatoli N.
AU - Baker, James R.
AU - Holl, Mark M.Banaszak
AU - Orr, Bradford G.
PY - 2009/8/13
Y1 - 2009/8/13
N2 - It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30-2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1-100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making ∼ 3 nm holes in living cell membranes.
AB - It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30-2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1-100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making ∼ 3 nm holes in living cell membranes.
UR - http://www.scopus.com/inward/record.url?scp=68549087087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68549087087&partnerID=8YFLogxK
U2 - 10.1021/jp9033936
DO - 10.1021/jp9033936
M3 - Article
C2 - 19606833
AN - SCOPUS:68549087087
VL - 113
SP - 11179
EP - 11185
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 32
ER -