Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells

Jae Sung Lim, Hyon E. Choy, Sang Chul Park, Jung Min Han, Ik Soon Jang, Kyung A. Cho

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolaedependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-β-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.

Original languageEnglish
Pages (from-to)243-251
Number of pages9
JournalAging Cell
Volume9
Issue number2
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Caveolae
Caveolin 1
Salmonella typhimurium
Salmonella
Endocytosis
Infection
Peyer's Patches
Salmonella Infections
Cyclodextrins
Eukaryotic Cells
Small Interfering RNA
Spleen

All Science Journal Classification (ASJC) codes

  • Ageing
  • Cell Biology

Cite this

Lim, Jae Sung ; Choy, Hyon E. ; Park, Sang Chul ; Han, Jung Min ; Jang, Ik Soon ; Cho, Kyung A. / Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells. In: Aging Cell. 2010 ; Vol. 9, No. 2. pp. 243-251.
@article{2ad4dd2cb1b14c9398ac03dd35108969,
title = "Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells",
abstract = "Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolaedependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-β-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.",
author = "Lim, {Jae Sung} and Choy, {Hyon E.} and Park, {Sang Chul} and Han, {Jung Min} and Jang, {Ik Soon} and Cho, {Kyung A.}",
year = "2010",
month = "4",
day = "1",
doi = "10.1111/j.1474-9726.2010.00554.x",
language = "English",
volume = "9",
pages = "243--251",
journal = "Aging Cell",
issn = "1474-9718",
publisher = "Wiley-Blackwell",
number = "2",

}

Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells. / Lim, Jae Sung; Choy, Hyon E.; Park, Sang Chul; Han, Jung Min; Jang, Ik Soon; Cho, Kyung A.

In: Aging Cell, Vol. 9, No. 2, 01.04.2010, p. 243-251.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells

AU - Lim, Jae Sung

AU - Choy, Hyon E.

AU - Park, Sang Chul

AU - Han, Jung Min

AU - Jang, Ik Soon

AU - Cho, Kyung A.

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolaedependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-β-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.

AB - Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolaedependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-β-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.

UR - http://www.scopus.com/inward/record.url?scp=77953267804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953267804&partnerID=8YFLogxK

U2 - 10.1111/j.1474-9726.2010.00554.x

DO - 10.1111/j.1474-9726.2010.00554.x

M3 - Article

C2 - 20096033

AN - SCOPUS:77953267804

VL - 9

SP - 243

EP - 251

JO - Aging Cell

JF - Aging Cell

SN - 1474-9718

IS - 2

ER -