Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells

Jae Sung Lim, Hyon E. Choy, Sang Chul Park, Jung Min Han, Ik Soon Jang, Kyung A. Cho

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolaedependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-β-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.

Original languageEnglish
Pages (from-to)243-251
Number of pages9
JournalAging Cell
Volume9
Issue number2
DOIs
Publication statusPublished - 2010 Apr

All Science Journal Classification (ASJC) codes

  • Ageing
  • Cell Biology

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