CD133+/C-kit+Lin endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia

Yejin Park, Hwa Jin Lee, Yun Ji Jung, Ha Yan Kwon, Heeyon Kim, Joon Ho Lee, Young Han Kim, Hyun Ok Kim, Yong Sun Maeng, Ja Young Kwon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: Individuals delivered from preeclamptic pregnancies exhibit a long-term increased risk of developing cardiovascular and metabolic diseases, likely caused by aberrant fetal cell reprogramming incurred in utero. The present study investigated the functional impairment and epigenetic changes exhibited by endothelial progenitor cells derived from offspring born to preeclamptic pregnancies. Study design: The capacity of CD133 + /C-kit + /Lin (CKL ) human umbilical cord blood endothelial progenitor cells (EPCs) derived from gestationally matched normal and preeclamptic (n = 10 each) pregnancies to differentiate to form outgrowth endothelial cells (OECs) was assessed by observing both their morphology, and the number and size of generated OECs colonies. Likewise, OECs angiogenic function was evaluated via migration, adhesion, and tube-formation assays. EPCs from preeclampsia were cultured in normal-, and preeclampsia-derived serum-conditioned media to assess the effects of environmental factors on EPC differentiation potency and OEC angiogenic function, and finally, EPCs H3K4, H3K9, and H3K27 trimethylation levels were assayed. Results: The preeclampsia-derived CKL EPCs exhibited decreased H3K4 and H3K9 trimethylation levels, significantly delayed differentiation times, and a significant reduction in both their number of generated OECs colonies, and exhibited reduced OECs migration, adhesion, and tube formation activities compared to those achieved by the normal-derived EPCs. Interestingly, the reduced differentiation potency of the preeclampsia-derived EPCs was not rescued via exposure to normal serum. Conclusions: Exposure to preeclampsia significantly and irreversibly reduced CKL EPC differentiation potency and OEC angiogenic function, likely reflecting incurred irreversible epigenetic changes.

Original languageEnglish
Pages (from-to)146-153
Number of pages8
JournalPregnancy Hypertension
Volume15
DOIs
Publication statusPublished - 2019 Jan

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Pre-Eclampsia
Endothelial Cells
Epigenomics
Pregnancy
Cell Differentiation
Endothelial Progenitor Cells
Metabolic Diseases
Conditioned Culture Medium
Serum
Fetal Blood
Cell Adhesion
Cell Movement
Blood Cells
Cardiovascular Diseases

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Obstetrics and Gynaecology

Cite this

Park, Yejin ; Lee, Hwa Jin ; Jung, Yun Ji ; Kwon, Ha Yan ; Kim, Heeyon ; Lee, Joon Ho ; Kim, Young Han ; Kim, Hyun Ok ; Maeng, Yong Sun ; Kwon, Ja Young. / CD133+/C-kit+Lin endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia In: Pregnancy Hypertension. 2019 ; Vol. 15. pp. 146-153.
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abstract = "Objectives: Individuals delivered from preeclamptic pregnancies exhibit a long-term increased risk of developing cardiovascular and metabolic diseases, likely caused by aberrant fetal cell reprogramming incurred in utero. The present study investigated the functional impairment and epigenetic changes exhibited by endothelial progenitor cells derived from offspring born to preeclamptic pregnancies. Study design: The capacity of CD133 + /C-kit + /Lin − (CKL − ) human umbilical cord blood endothelial progenitor cells (EPCs) derived from gestationally matched normal and preeclamptic (n = 10 each) pregnancies to differentiate to form outgrowth endothelial cells (OECs) was assessed by observing both their morphology, and the number and size of generated OECs colonies. Likewise, OECs angiogenic function was evaluated via migration, adhesion, and tube-formation assays. EPCs from preeclampsia were cultured in normal-, and preeclampsia-derived serum-conditioned media to assess the effects of environmental factors on EPC differentiation potency and OEC angiogenic function, and finally, EPCs H3K4, H3K9, and H3K27 trimethylation levels were assayed. Results: The preeclampsia-derived CKL − EPCs exhibited decreased H3K4 and H3K9 trimethylation levels, significantly delayed differentiation times, and a significant reduction in both their number of generated OECs colonies, and exhibited reduced OECs migration, adhesion, and tube formation activities compared to those achieved by the normal-derived EPCs. Interestingly, the reduced differentiation potency of the preeclampsia-derived EPCs was not rescued via exposure to normal serum. Conclusions: Exposure to preeclampsia significantly and irreversibly reduced CKL − EPC differentiation potency and OEC angiogenic function, likely reflecting incurred irreversible epigenetic changes.",
author = "Yejin Park and Lee, {Hwa Jin} and Jung, {Yun Ji} and Kwon, {Ha Yan} and Heeyon Kim and Lee, {Joon Ho} and Kim, {Young Han} and Kim, {Hyun Ok} and Maeng, {Yong Sun} and Kwon, {Ja Young}",
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CD133+/C-kit+Lin endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia . / Park, Yejin; Lee, Hwa Jin; Jung, Yun Ji; Kwon, Ha Yan; Kim, Heeyon; Lee, Joon Ho; Kim, Young Han; Kim, Hyun Ok; Maeng, Yong Sun; Kwon, Ja Young.

In: Pregnancy Hypertension, Vol. 15, 01.2019, p. 146-153.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CD133+/C-kit+Lin − endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia

AU - Park, Yejin

AU - Lee, Hwa Jin

AU - Jung, Yun Ji

AU - Kwon, Ha Yan

AU - Kim, Heeyon

AU - Lee, Joon Ho

AU - Kim, Young Han

AU - Kim, Hyun Ok

AU - Maeng, Yong Sun

AU - Kwon, Ja Young

PY - 2019/1

Y1 - 2019/1

N2 - Objectives: Individuals delivered from preeclamptic pregnancies exhibit a long-term increased risk of developing cardiovascular and metabolic diseases, likely caused by aberrant fetal cell reprogramming incurred in utero. The present study investigated the functional impairment and epigenetic changes exhibited by endothelial progenitor cells derived from offspring born to preeclamptic pregnancies. Study design: The capacity of CD133 + /C-kit + /Lin − (CKL − ) human umbilical cord blood endothelial progenitor cells (EPCs) derived from gestationally matched normal and preeclamptic (n = 10 each) pregnancies to differentiate to form outgrowth endothelial cells (OECs) was assessed by observing both their morphology, and the number and size of generated OECs colonies. Likewise, OECs angiogenic function was evaluated via migration, adhesion, and tube-formation assays. EPCs from preeclampsia were cultured in normal-, and preeclampsia-derived serum-conditioned media to assess the effects of environmental factors on EPC differentiation potency and OEC angiogenic function, and finally, EPCs H3K4, H3K9, and H3K27 trimethylation levels were assayed. Results: The preeclampsia-derived CKL − EPCs exhibited decreased H3K4 and H3K9 trimethylation levels, significantly delayed differentiation times, and a significant reduction in both their number of generated OECs colonies, and exhibited reduced OECs migration, adhesion, and tube formation activities compared to those achieved by the normal-derived EPCs. Interestingly, the reduced differentiation potency of the preeclampsia-derived EPCs was not rescued via exposure to normal serum. Conclusions: Exposure to preeclampsia significantly and irreversibly reduced CKL − EPC differentiation potency and OEC angiogenic function, likely reflecting incurred irreversible epigenetic changes.

AB - Objectives: Individuals delivered from preeclamptic pregnancies exhibit a long-term increased risk of developing cardiovascular and metabolic diseases, likely caused by aberrant fetal cell reprogramming incurred in utero. The present study investigated the functional impairment and epigenetic changes exhibited by endothelial progenitor cells derived from offspring born to preeclamptic pregnancies. Study design: The capacity of CD133 + /C-kit + /Lin − (CKL − ) human umbilical cord blood endothelial progenitor cells (EPCs) derived from gestationally matched normal and preeclamptic (n = 10 each) pregnancies to differentiate to form outgrowth endothelial cells (OECs) was assessed by observing both their morphology, and the number and size of generated OECs colonies. Likewise, OECs angiogenic function was evaluated via migration, adhesion, and tube-formation assays. EPCs from preeclampsia were cultured in normal-, and preeclampsia-derived serum-conditioned media to assess the effects of environmental factors on EPC differentiation potency and OEC angiogenic function, and finally, EPCs H3K4, H3K9, and H3K27 trimethylation levels were assayed. Results: The preeclampsia-derived CKL − EPCs exhibited decreased H3K4 and H3K9 trimethylation levels, significantly delayed differentiation times, and a significant reduction in both their number of generated OECs colonies, and exhibited reduced OECs migration, adhesion, and tube formation activities compared to those achieved by the normal-derived EPCs. Interestingly, the reduced differentiation potency of the preeclampsia-derived EPCs was not rescued via exposure to normal serum. Conclusions: Exposure to preeclampsia significantly and irreversibly reduced CKL − EPC differentiation potency and OEC angiogenic function, likely reflecting incurred irreversible epigenetic changes.

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