CD24+ cells from hierarchically organized ovarian cancer are enriched in cancer stem cells

M. Q. Gao, Y. P. Choi, S. Kang, J. H. Youn, N. H. Cho

Research output: Contribution to journalArticle

253 Citations (Scopus)

Abstract

Cancer stem cells (CSCs) have been identified in solid tumors and cancer cell lines. In this study, we isolated a series of cancer cell clones, which were heterogeneous in growth rate, cell cycle distribution and expression profile of genes and proteins, from ovarian tumor specimens of a patient and identified a sub-population enriched for ovarian CSCs defined by CD24 phenotype. Experiments in vitro demonstrated CD24+ sub-population possessed stem cell-like characteristics of remaining quiescence and more chemoresistant compared with CD24- fraction, as well as a specific capacity for self-renewal and differentiation. In addition, injection of 5 × 10 3 CD24+ cells was able to form tumor xenografts in nude mice, whereas equal number of CD24- cells remained nontumorigenic. We also found that CD24+ cells expressed higher mRNA levels of some stemness genes, including Nestin, Β-catenin, Bmi-1, Oct4, Oct3/4, Notch1 and Notch4 which were involved in modulating many functions of stem cells, and lower E-cadherin mRNA level than CD24 cells. Altogether, these observations suggest human ovarian tumor cells are organized as a hierarchy and CD24 demarcates an ovarian cancer-initiating cell population. These findings will have important clinical applications for developing effective therapeutic strategies to treat ovarian cancer.

Original languageEnglish
Pages (from-to)2672-2680
Number of pages9
JournalOncogene
Volume29
Issue number18
DOIs
Publication statusPublished - 2010 May 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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