cDNA cloning, expression, and chromosomal localization of Caenorhabditis elegans DNA topoisomerase I

Jonghwa Kim, You Chan Kim, Jong Hyouk Lee, Yeon Joo Jang, In Kwon Chung, Hyeon Sook Koo

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Abstract

By screening Caenorhabditis elegans cDNA libraries, overlapping cDNA clones encoding DNA topoisomerase I were obtained. An open reading frame of 751 amino acids was found in the 3.2-kb cDNA sequence. The open reading frame has 54% and 50% identities to the amino acid sequences of human and Drosophila melanogaster DNA topoisomerases I, respectively. Northern blot analysis showed the presence of an mRNA of 3.4 kb which suggests that the cDNA sequence is close to full length. The 72-kDa C-terminal polypeptide expressed in Escherichia coli cells showed catalytic DNA topoisomerase I activity. The DNA topoisomerase I gene was mapped to position 18 of chromosome I by screening polytene YAC plasmid DNAs.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalEuropean Journal of Biochemistry
Volume237
Issue number2
DOIs
Publication statusPublished - 1996 Jan 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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