cDNA cloning, expression, and chromosomal localization of Caenorhabditis elegans DNA topoisomerase I

Jonghwa Kim, You Chan Kim, Jong Hyouk Lee, Yeon Joo Jang, In Kwon Chung, Hyeon-Sook Koo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

By screening Caenorhabditis elegans cDNA libraries, overlapping cDNA clones encoding DNA topoisomerase I were obtained. An open reading frame of 751 amino acids was found in the 3.2-kb cDNA sequence. The open reading frame has 54% and 50% identities to the amino acid sequences of human and Drosophila melanogaster DNA topoisomerases I, respectively. Northern blot analysis showed the presence of an mRNA of 3.4 kb which suggests that the cDNA sequence is close to full length. The 72-kDa C-terminal polypeptide expressed in Escherichia coli cells showed catalytic DNA topoisomerase I activity. The DNA topoisomerase I gene was mapped to position 18 of chromosome I by screening polytene YAC plasmid DNAs.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalEuropean Journal of Biochemistry
Volume237
Issue number2
DOIs
Publication statusPublished - 1996 Jan 1

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Type I DNA Topoisomerase
Cloning
Caenorhabditis elegans
Organism Cloning
Complementary DNA
Open Reading Frames
Screening
Catalytic DNA
Chromosomes, Human, Pair 18
Amino Acids
Chromosomes
Drosophila melanogaster
Gene Library
Northern Blotting
Escherichia coli
Amino Acid Sequence
Plasmids
Clone Cells
Genes
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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abstract = "By screening Caenorhabditis elegans cDNA libraries, overlapping cDNA clones encoding DNA topoisomerase I were obtained. An open reading frame of 751 amino acids was found in the 3.2-kb cDNA sequence. The open reading frame has 54{\%} and 50{\%} identities to the amino acid sequences of human and Drosophila melanogaster DNA topoisomerases I, respectively. Northern blot analysis showed the presence of an mRNA of 3.4 kb which suggests that the cDNA sequence is close to full length. The 72-kDa C-terminal polypeptide expressed in Escherichia coli cells showed catalytic DNA topoisomerase I activity. The DNA topoisomerase I gene was mapped to position 18 of chromosome I by screening polytene YAC plasmid DNAs.",
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cDNA cloning, expression, and chromosomal localization of Caenorhabditis elegans DNA topoisomerase I. / Kim, Jonghwa; Kim, You Chan; Lee, Jong Hyouk; Jang, Yeon Joo; Chung, In Kwon; Koo, Hyeon-Sook.

In: European Journal of Biochemistry, Vol. 237, No. 2, 01.01.1996, p. 367-372.

Research output: Contribution to journalArticle

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