Cell cycle-dependent expression of cIAP2 at G2/M phase contributes to survival during mitotic cell cycle arrest

Hyung Seung Jin, Tae H. Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

cIAP2 (cellular inhibitor of apoptosis protein 2) is induced by NF-κB (nuclear factor κB) when cells need to respond quickly to different apoptotic stimuli. A recent study using cDNA microarray technology has suggested that cIAP2 transcription is regulated in a cell cycle-dependent manner, although the mechanism for such regulation is unknown. In this study, we confirmed the cell cycle-dependent regulation of cIAP2 expression at both the mRNA and protein levels. Additionally, we found that a bipartite CDE (cell cycle-dependent element)/CHR (cell cycle gene homology region) element in the cIAP2 promoter mediates cIAP2 gene activation in G2/M phase. Cell cycle-dependent G2/M-phase-specific cIAP2 expression is enhanced by NF-κB activation, and selective down-regulation of cIAP2 causes cells blocked in mitosis with nocodazole to become susceptible to apoptosis, indicating that the G2/M-phase-specific expression of cIAP2 contributes to the survival of mitotically arrested cells. Our studies describing the NF-κB-independent G2/M-phase-specific expression of cIAP2 will help in further understanding the molecular basis of cIAP2 overexpression in a variety of human cancers.

Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalBiochemical Journal
Volume399
Issue number2
DOIs
Publication statusPublished - 2006 Oct 15

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Inhibitor of Apoptosis Proteins
G2 Phase
Cell Cycle Checkpoints
Cell Division
Cell Cycle
Cells
Survival
Genes
Chemical activation
Nocodazole
cdc Genes
Transcription
Microarrays
Oligonucleotide Array Sequence Analysis
Mitosis
Transcriptional Activation
Down-Regulation
Complementary DNA
Apoptosis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Cell cycle-dependent expression of cIAP2 at G2/M phase contributes to survival during mitotic cell cycle arrest. / Jin, Hyung Seung; Lee, Tae H.

In: Biochemical Journal, Vol. 399, No. 2, 15.10.2006, p. 335-342.

Research output: Contribution to journalArticle

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