Cell cycle of the enamel knot during tooth morphogenesis

Seo Yoon Jung, David William Green, Han Sung Jung, Eun Jung Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Enamel knot (EK) is known to be a central organ in tooth development, especially for cusp patterning. To trace the exact position and movement among the inner dental epithelium (IDE) and EK cells, and to monitor the relationship between the EK and cusp patterning, it is essential that we understand the cell cycle status of the EK in early stages of tooth development. In this study, thymidine analogous (IdU, BrdU) staining was used to evaluate the cell cycle phase of the primary EK at the early casp stage (E13.0) and the gerbil embryo (E19) in a developing mouse embryo. The centerpiece of this study was to describe the cell cycle phasing and sequencing during proliferation in the IDE according to the expression of IdU and BrdU following their injection at calculated time points. The interval time between IdU injection and BrdU injection was set at 4 h. As a result, the cell cycle in the IDE of the mouse and gerbil was found to be synchronous. Conversely, the cell cycle in primary EKs of mice was much longer than that of the IDE. Therefore, the difference of cell cycle of the IDE and the EK is related to the diversity of cusp patterning and would provide a new insight into tooth morphogenesis.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalHistochemistry and cell biology
Issue number6
Publication statusPublished - 2018 Jun 1

Bibliographical note

Funding Information:
Acknowledgements We are grateful to Chengri Li for helping experiments. This study was supported by the Yonsei University College of Dentistry (6-2016-0021).

Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology


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