Cell fate in the Arabidopsis root epidermis is determined by competition between WEREWOLF and CAPRICE

Sang Kee Song, Kook Hui Ryu, Yeon Hee Kang, Jae Hyo Song, Young Hee Cho, Sang Dong Yoo, John Schiefelbein, Myeong Min Lee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The root hair and nonhair cells in the Arabidopsis (Arabidopsis thaliana) root epidermis are specified by a suite of transcriptional regulators. Two of these are WEREWOLF (WER) and CAPRICE (CPC), which encode MYB transcription factors that are required for promoting the nonhair cell fate and the hair cell fate, respectively. However, the precise function and relationship between these transcriptional regulators have not been fully defined experimentally. Here, we examine these issues by misexpressing the WER gene using the GAL4-upstream activation sequence transactivation system. We find that WER overexpression in the Arabidopsis root tip is sufficient to cause epidermal cells to adopt the nonhair cell fate through direct induction of GLABRA2 (GL2) gene expression.We also show that GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3), two closely related bHLH proteins, are required for the action of the overexpressed WER and that WER interacts with these bHLHs in plant cells. Furthermore, we find that CPC suppresses the WER overexpression phenotype quantitatively. These results show that WER acts together with GL3/EGL3 to induce GL2 expression and that WER and CPC compete with one another to define cell fates in the Arabidopsis root epidermis,

Original languageEnglish
Pages (from-to)1196-1208
Number of pages13
JournalPlant physiology
Volume157
Issue number3
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

epidermis (animal)
Arabidopsis
Epidermis
cells
transcription factors
Basic Helix-Loop-Helix Transcription Factors
Meristem
Plant Cells
root hairs
Transcriptional Activation
transcriptional activation
epidermis (plant)
root tips
trichomes
Transcription Factors
Arabidopsis thaliana
Phenotype
Gene Expression
phenotype
gene expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Song, Sang Kee ; Ryu, Kook Hui ; Kang, Yeon Hee ; Song, Jae Hyo ; Cho, Young Hee ; Yoo, Sang Dong ; Schiefelbein, John ; Lee, Myeong Min. / Cell fate in the Arabidopsis root epidermis is determined by competition between WEREWOLF and CAPRICE. In: Plant physiology. 2011 ; Vol. 157, No. 3. pp. 1196-1208.
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abstract = "The root hair and nonhair cells in the Arabidopsis (Arabidopsis thaliana) root epidermis are specified by a suite of transcriptional regulators. Two of these are WEREWOLF (WER) and CAPRICE (CPC), which encode MYB transcription factors that are required for promoting the nonhair cell fate and the hair cell fate, respectively. However, the precise function and relationship between these transcriptional regulators have not been fully defined experimentally. Here, we examine these issues by misexpressing the WER gene using the GAL4-upstream activation sequence transactivation system. We find that WER overexpression in the Arabidopsis root tip is sufficient to cause epidermal cells to adopt the nonhair cell fate through direct induction of GLABRA2 (GL2) gene expression.We also show that GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3), two closely related bHLH proteins, are required for the action of the overexpressed WER and that WER interacts with these bHLHs in plant cells. Furthermore, we find that CPC suppresses the WER overexpression phenotype quantitatively. These results show that WER acts together with GL3/EGL3 to induce GL2 expression and that WER and CPC compete with one another to define cell fates in the Arabidopsis root epidermis,",
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Song, SK, Ryu, KH, Kang, YH, Song, JH, Cho, YH, Yoo, SD, Schiefelbein, J & Lee, MM 2011, 'Cell fate in the Arabidopsis root epidermis is determined by competition between WEREWOLF and CAPRICE', Plant physiology, vol. 157, no. 3, pp. 1196-1208. https://doi.org/10.1104/pp.111.185785

Cell fate in the Arabidopsis root epidermis is determined by competition between WEREWOLF and CAPRICE. / Song, Sang Kee; Ryu, Kook Hui; Kang, Yeon Hee; Song, Jae Hyo; Cho, Young Hee; Yoo, Sang Dong; Schiefelbein, John; Lee, Myeong Min.

In: Plant physiology, Vol. 157, No. 3, 01.11.2011, p. 1196-1208.

Research output: Contribution to journalArticle

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AU - Ryu, Kook Hui

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AU - Lee, Myeong Min

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